On sampling procedures for detection of Heterodera glycines, the soybean cyst nematode, and other soil dwelling organisms

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dc.contributor.author McLellan, Alexander
dc.date.accessioned 2016-08-12T20:26:07Z
dc.date.available 2016-08-12T20:26:07Z
dc.date.issued 2016-08-01 en_US
dc.identifier.uri http://hdl.handle.net/2097/32915
dc.description.abstract Heterodera glycines, or the soybean cyst nematode (SCN), is a parasite that targets and damages the roots of soybean plants. It is the most yield-limiting pathogen of soybean in the U.S. and the reliable detection and accurate estimation of population densities is crucial to research and management of this pathogen. A study was performed to understand the effects of crop rotation on the prevalence of SCN. Standard sampling procedures in the plant pathology community dictate taking soil samples from potentially infected fields, processing them and counting the number of eggs in one 1 mL subsample via microscope. Suspecting the traditional procedure may lead to invalid results, false negatives in particular, the researcher created and implemented a sampling procedure based on his knowledge of sampling methods and constraints of sampling in the field. Using the data collected, we will discuss the strengths and limitations of the procedure in estimating the population density of SCN in the field. In addition, a simulation study informed by the data will be conducted to determine a sampling strategy that will yield accurate results while still considering the conditions in the field. Knowledge on how the different stages of the sampling procedure for SCN affect the accurate detection of the pathogen would extend to experimental designs and sampling methodologies for other soil dwelling organisms. en_US
dc.language.iso en_US en_US
dc.publisher Kansas State University en
dc.subject Heterodera glycines en_US
dc.subject Soybean Cyst Nematode en_US
dc.subject Spatial statistics en_US
dc.subject Simulation study en_US
dc.subject Sampling procedures en_US
dc.subject Plant parasite en_US
dc.title On sampling procedures for detection of Heterodera glycines, the soybean cyst nematode, and other soil dwelling organisms en_US
dc.type Report en_US
dc.description.degree Master of Science en_US
dc.description.level Masters en_US
dc.description.department Department of Statistics en_US
dc.description.advisor Perla Reyes en_US
dc.date.published 2016 en_US
dc.date.graduationmonth August en_US

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