Identification and deployment of QTL for Fusarium head blight resistance in U.S. hard winter wheat

K-REx Repository

Show simple item record

dc.contributor.author Fatima, Nosheen
dc.date.accessioned 2016-04-25T15:36:39Z
dc.date.available 2016-04-25T15:36:39Z
dc.date.issued 2016-05-01 en_US
dc.identifier.uri http://hdl.handle.net/2097/32679
dc.description.abstract Fusarium head blight (FHB) is one of the most damaging diseases in wheat, which impacts both grain yield and quality drastically. Recently, the disease has become more prevalent in the hard winter wheat (HWW) grown areas of the United States including Oklahoma where FHB has not been reported before. Growing resistant cultivars is the most economical and effective strategy for disease management. To dissect quantitative trait loci (QTL) for FHB resistance in a moderately resistant hard winter wheat (HWW) cultivar, Overland, a population of 186 recombinant inbred lines (RILs) was developed from the cross between Overland and Overley, a susceptible HWW cultivar from Kansas. The RILs were evaluated for FHB type II resistance in one field and three greenhouse experiments and genotyped using genotyping-by-sequencing (GBS) markers. Three FHB resistance QTLs were mapped on Chromosomes 4DL, 4AL, and 5BL. The QTL on 4DL was the most consistent one and explained up to 13% of the phenotypic variation for type II resistance and 14 % for low Fusarium damaged kernels (FDK). Two GBS markers closely linked to the 4DL QTL were successfully converted to Kbioscience competitive allelic specific PCR (KASP) assays and can be used in marker-assisted breeding. In breeding, a single QTL may provide only partial resistance and pyramiding of several resistance QTLs in a cultivar can provide more protection in FHB epidemics. Fhb1 is a major QTL for FHB resistance from a Chinese source and Fhb3 is an alien gene from wild rye grass (Leymus racemosus). To study the effects of these QTLs individually and cumulatively in hard winter wheat backgrounds, they were transferred into two HWW cultivars Overland and Jagger. The results show that Fhb1 significantly increased FHB resistance, but Fhb3 did not. Thus, Fhb3 is not an effective gene for improvement of FHB resistance in HWW. en_US
dc.description.sponsorship U.S. Wheat and Barley scab initiative Fulbright Scholarship en_US
dc.language.iso en_US en_US
dc.publisher Kansas State University en
dc.subject QTL mapping in U.S. winter wheat en_US
dc.title Identification and deployment of QTL for Fusarium head blight resistance in U.S. hard winter wheat en_US
dc.type Thesis en_US
dc.description.degree Master of Science en_US
dc.description.level Masters en_US
dc.description.department Agronomy en_US
dc.description.advisor Guihua Bai en_US
dc.description.advisor Guorong Zhang en_US
dc.date.published 2016 en_US
dc.date.graduationmonth May en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search K-REx


Advanced Search

Browse

My Account

Statistics








Center for the

Advancement of Digital

Scholarship

cads@k-state.edu