Spatial differentiation of gene expression in Aspergillus niger colony grown for sugar beet pulp utilization

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dc.contributor.author Benoit, I.
dc.contributor.author Zhou, M. M.
dc.contributor.author Duarte, A. V.
dc.contributor.author Downes, D. J.
dc.contributor.author Todd, Richard B.
dc.contributor.author Kloezen, W.
dc.contributor.author Post, H.
dc.contributor.author Heck, A. J. R.
dc.contributor.author Altelaar, A. F. M.
dc.contributor.author de Vries, R. P.
dc.date.accessioned 2016-04-06T15:14:25Z
dc.date.available 2016-04-06T15:14:25Z
dc.identifier.uri http://hdl.handle.net/2097/32464
dc.description Citation: Benoit, I., Zhou, M. M., Duarte, A. V., Downes, D. J., Todd, R. B., Kloezen, W., . . . de Vries, R. P. (2015). Spatial differentiation of gene expression in Aspergillus niger colony grown for sugar beet pulp utilization. Scientific Reports, 5, 13. doi:10.1038/srep13592
dc.description Degradation of plant biomass to fermentable sugars is of critical importance for the use of plant materials for biofuels. Filamentous fungi are ubiquitous organisms and major plant biomass degraders. Single colonies of some fungal species can colonize massive areas as large as five soccer stadia. During growth, the mycelium encounters heterogeneous carbon sources. Here we assessed whether substrate heterogeneity is a major determinant of spatial gene expression in colonies of Aspergillus niger. We analyzed whole-genome gene expression in five concentric zones of 5-day-old colonies utilizing sugar beet pulp as a complex carbon source. Growth, protein production and secretion occurred throughout the colony. Genes involved in carbon catabolism were expressed uniformly from the centre to the periphery whereas genes encoding plant biomass degrading enzymes and nitrate utilization were expressed differentially across the colony. A combined adaptive response of carbon-catabolism and enzyme production to locally available monosaccharides was observed. Finally, our results demonstrate that A. niger employs different enzymatic tools to adapt its metabolism as it colonizes complex environments.
dc.relation.uri https://doi.org/10.1038/srep13592
dc.rights Attribution 4.0 International (CC BY 4.0)
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject Ammonium Permease Genes
dc.subject Signal Peptides
dc.subject Microarray Data
dc.subject Proteomics
dc.subject Data
dc.subject Nidulans
dc.title Spatial differentiation of gene expression in Aspergillus niger colony grown for sugar beet pulp utilization
dc.type Article
dc.date.published 2015
dc.citation.doi 10.1038/srep13592
dc.citation.issn 2045-2322
dc.citation.jtitle Scientific Reports
dc.citation.spage 13
dc.citation.volume 5
dc.contributor.authoreid rbtodd


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