Genetic structuring and fixed polymorphisms in the gene period among natural populations of Lutzomyia longipalpis in Brazil

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dc.contributor.author Costa, C. R. L.
dc.contributor.author Freitas, M. T. D.
dc.contributor.author Figueiredo, C. A. S.
dc.contributor.author Aragao, N. C.
dc.contributor.author da Silva, L. G.
dc.contributor.author Marcondes, C. B.
dc.contributor.author Dias, R. V.
dc.contributor.author Leal-Balbino, T. C.
dc.contributor.author Souza, M. B. R.
dc.contributor.author Ramalho-Ortigao, Marcelo
dc.contributor.author Balbino, V. D.
dc.date.accessioned 2016-04-04T22:46:00Z
dc.date.available 2016-04-04T22:46:00Z
dc.identifier.uri http://hdl.handle.net/2097/32304
dc.description Citation: Costa, C. R. L., Freitas, M. T. D., Figueiredo, C. A. S., Aragao, N. C., da Silva, L. G., Marcondes, C. B., . . . Balbino, V. D. (2015). Genetic structuring and fixed polymorphisms in the gene period among natural populations of Lutzomyia longipalpis in Brazil. Parasites & Vectors, 8, 9. doi:10.1186/s13071-015-0785-6
dc.description Background: Even one hundred years after being originally identified, aspects of the taxonomy of the sand fly Lutzomyia longipalpis, the principal vector of Leishmania infantum in the Americas, remain unresolved for Brazilian populations of this vector. The diversity of morphological, behavioral, biochemical, and ethological characters, as well as the genetic variability detected by molecular markers are indicative of the presence of a complex of species. Methods: In this study, a 525 bp fragment of the period gene was used to evaluate sympatric populations of L. longipalpis. A combination of probabilistic methods such as maximum likelihood and genetic assignment approach to investigate sympatric species of L. longipalpis were applied in three populations of Northeast Brazil. Results: Fixed polymorphisms in geographically isolated populations of L. longipalpis from two localities in the state of Ceara and one in the state of Pernambuco, Brazil, was identified in a 525 bp fragment of the gene period (per). Our results suggest a direct relationship between the number of spots found in males' tergites and the genetic variation in cryptic species of L. longipalpis. The fragment used in this study revealed the nature of the ancestral morphotype 1S. Conclusion: New polymorphisms were identified in the gene per which can be used as a genetic barcode to sympatric taxonomy of L. longipalpis. The per gene fragment confirmed the presence of two siblings species of L. longipalpis in Sobral and showed that these same species are present in two other localities, representing an expansion within the L. longipalpis species complex with regards to the states of Ceara and Pernambuco.
dc.relation.uri https://doi.org/10.1186/s13071-015-0785-6
dc.rights Attribution 4.0 International (CC BY 4.0)
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject Lutzomyia Longipalpis Complex
dc.subject Period Gene
dc.subject Fixed Polymorphism
dc.subject American Visceral Leishmaniasis
dc.subject Microsatellite Markers
dc.subject Molecular
dc.title Genetic structuring and fixed polymorphisms in the gene period among natural populations of Lutzomyia longipalpis in Brazil
dc.type Article
dc.date.published 2015
dc.citation.doi 10.1186/s13071-015-0785-6
dc.citation.issn 1756-3305
dc.citation.issue 1
dc.citation.jtitle Parasites & Vectors
dc.citation.spage 9
dc.citation.volume 8
dc.contributor.authoreid mortigao


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