Unbalanced Activation of Glutathione Metabolic Pathways Suggests Potential Involvement in Plant Defense against the Gall Midge Mayetiola destructor in Wheat

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dc.contributor.author Liu, Xuming M.
dc.contributor.author Zhang, S. Z.
dc.contributor.author Whitworth, R. J.
dc.contributor.author Stuart, J. J.
dc.contributor.author Chen, M. S.
dc.date.accessioned 2016-04-04T22:44:42Z
dc.date.available 2016-04-04T22:44:42Z
dc.identifier.uri http://hdl.handle.net/2097/32271
dc.description Citation: Liu, X. M., Zhang, S. Z., Whitworth, R. J., Stuart, J. J., & Chen, M. S. (2015). Unbalanced Activation of Glutathione Metabolic Pathways Suggests Potential Involvement in Plant Defense against the Gall Midge Mayetiola destructor in Wheat. Scientific Reports, 5, 7. doi:10.1038/srep08092
dc.description Glutathione, gamma-glutamylcysteinylglycine, exists abundantly in nearly all organisms. Glutathione participates in various physiological processes involved in redox reactions by serving as an electron donor/acceptor. We found that the abundance of total glutathione increased up to 60% in resistant wheat plants within 72 hours following attack by the gall midge Mayetiola destructor, the Hessian fly. The increase in total glutathione abundance, however, is coupled with an unbalanced activation of glutathione metabolic pathways. The activity and transcript abundance of glutathione peroxidases, which convert reduced glutathione (GSH) to oxidized glutathione (GSSG), increased in infested resistant plants. However, the enzymatic activity and transcript abundance of glutathione reductases, which convert GSSG back to GSH, did not change. This unbalanced regulation of the glutathione oxidation/reduction cycle indicates the existence of an alternative pathway to regenerate GSH from GSSG to maintain a stable GSSG/GSH ratio. Our data suggest the possibility that GSSG is transported from cytosol to apoplast to serve as an oxidant for class III peroxidases to generate reactive oxygen species for plant defense against Hessian fly larvae. Our results provide a foundation for elucidating the molecular processes involved in glutathione-mediated plant resistance to Hessian fly and potentially other pests as well.
dc.relation.uri https://doi.org/10.1038/srep08092
dc.rights Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subject Apoplastic Oxidative Burst
dc.subject Hessian Fly Diptera
dc.subject Gene-Expression
dc.subject Hypersensitive Response
dc.subject Disease Resistance
dc.subject S-Transferases
dc.title Unbalanced Activation of Glutathione Metabolic Pathways Suggests Potential Involvement in Plant Defense against the Gall Midge Mayetiola destructor in Wheat
dc.type Article
dc.date.published 2015
dc.citation.doi 10.1038/srep08092
dc.citation.issn 2045-2322
dc.citation.jtitle Scientific Reports
dc.citation.spage 7
dc.citation.volume 5
dc.contributor.authoreid xmliu


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