Microstencils to generate defined, multi-species patterns of bacteria

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dc.contributor.author Timm, C. M.
dc.contributor.author Hansen, Ryan R.
dc.contributor.author Doktycz, M. J.
dc.contributor.author Retterer, S. T.
dc.contributor.author Pelletier, D. A.
dc.date.accessioned 2016-04-04T22:20:26Z
dc.date.available 2016-04-04T22:20:26Z
dc.identifier.uri http://hdl.handle.net/2097/32254
dc.description Citation: Timm, C. M., Hansen, R. R., Doktycz, M. J., Retterer, S. T., & Pelletier, D. A. (2015). Microstencils to generate defined, multi-species patterns of bacteria. Biomicrofluidics, 9(6). doi:10.1063/1.4935938
dc.description Microbial communities are complex heterogeneous systems that are influenced by physical and chemical interactions with their environment, host, and community members. Techniques that facilitate the quantitative evaluation of how microscale organization influences the morphogenesis of multispecies communities could provide valuable insights into the dynamic behavior and organization of natural communities, the design of synthetic environments for multispecies culture, and the engineering of artificial consortia. In this work, we demonstrate a method for patterning microbes into simple arrangements that allow the quantitative measurement of growth dynamics as a function of their proximity to one another. The method combines parylene-based liftoff techniques with microfluidic delivery to simultaneously pattern multiple bacterial species with high viability using low-cost, customizable methods. Quantitative measurements of bacterial growth for two competing isolates demonstrate that spatial coordination can play a critical role in multispecies growth and structure. © 2015 AIP Publishing LLC.
dc.relation.uri https://doi.org/10.1063/1.4935938
dc.rights Copyright 2015 AIP Publishing. This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. The following article appeared in Biomicrofluidics, Vol. 9, 064103 (2015) and may be found at https://doi.org/10.1063/1.4935938.
dc.rights.uri http://www.sherpa.ac.uk/romeo/issn/1932-1058/
dc.subject Microorganisms
dc.subject Chemical Interactions
dc.subject Complex Heterogeneous Systems
dc.subject Microbial Communities
dc.subject Microfluidic Delivery
dc.subject Multi-Species Communities
dc.title Microstencils to generate defined, multi-species patterns of bacteria
dc.type Article
dc.date.published 2015
dc.citation.doi 10.1063/1.4935938
dc.citation.issn 1932-1058
dc.citation.issue 6
dc.citation.jtitle Biomicrofluidics
dc.citation.volume 9
dc.contributor.authoreid rrhansen


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