Reciprocal and dynamic polarization of planar cell polarity core components and myosin

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dc.contributor.author Newman-Smith, E.
dc.contributor.author Kourakis, M. J.
dc.contributor.author Reeves, W.
dc.contributor.author Veeman, Michael T.
dc.contributor.author Smith, W. C.
dc.date.accessioned 2016-04-04T22:13:44Z
dc.date.available 2016-04-04T22:13:44Z
dc.identifier.uri http://hdl.handle.net/2097/32227
dc.description Citation: Newman-Smith, E., Kourakis, M. J., Reeves, W., Veeman, M., & Smith, W. C. (2015). Reciprocal and dynamic polarization of planar cell polarity core components and myosin. eLife, 2015(4). doi:10.7554/eLife.05361
dc.description The Ciona notochord displays PCP-dependent polarity, with anterior localization of Prickle (Pk) and Strabismus (Stbm). We report that a myosin is polarized anteriorly in these cells and strongly colocalize with Stbm. Disruption of the actin/myosin machinery with cytochalasin or blebbistatin disrupts polarization of Pk and Stbm, but not of myosin complexes, suggesting a PCP-independent aspect of myosin localization. Washout of cytochalasin restored Pk polarization, but not if done in the presence of blebbistatin, suggesting an active role for myosin in core PCP protein localization. On the other hand, in the pk mutant line aimless myosin polarization in approximately one third of the cells, indicating a reciprocal action of core PCP signaling on myosin localization. Our results indicate a complex relationship between the actomyosin cytoskeleton and core PCP components in which myosin is not simply a downstream target of PCP signaling, but also required for PCP protein localization. © 2015, eLife. All rights reserved.
dc.relation.uri https://doi.org/10.7554/eLife.05361
dc.rights Attribution 4.0 International (CC BY 4.0)
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject Actin
dc.subject Blebbistatin
dc.subject Cytochalasin B
dc.subject Ensconsin
dc.subject Green Fluorescent Protein
dc.subject Latrunculin B
dc.title Reciprocal and dynamic polarization of planar cell polarity core components and myosin
dc.type Article
dc.date.published 2015
dc.citation.doi 10.7554/eLife.05361
dc.citation.issn 2050-084X
dc.citation.issue 4
dc.citation.jtitle eLife
dc.citation.volume 2015
dc.contributor.authoreid veeman


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