A 3D searchable database of transgenic zebrafish gal4 and cre lines for functional neuroanatomy studies

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dc.contributor.author Marquart, G. D.
dc.contributor.author Tabor, K. M.
dc.contributor.author Brown, M.
dc.contributor.author Strykowski, J. L.
dc.contributor.author Varshney, G. K.
dc.contributor.author LaFave, M. C.
dc.contributor.author Mueller, Thomas
dc.contributor.author Burgess, S. M.
dc.contributor.author Higashijima, S. I.
dc.contributor.author Burgess, H. A.
dc.date.accessioned 2016-04-04T22:13:43Z
dc.date.available 2016-04-04T22:13:43Z
dc.identifier.uri http://hdl.handle.net/2097/32225
dc.description Citation: Marquart, G. D., Tabor, K. M., Brown, M., Strykowski, J. L., Varshney, G. K., LaFave, M. C., . . . Burgess, H. A. (2015). A 3D searchable database of transgenic zebrafish gal4 and cre lines for functional neuroanatomy studies. Frontiers in Neural Circuits, 9(November), 1-17. doi:10.3389/fncir.2015.00078
dc.description Transgenic methods enable the selective manipulation of neurons for functional mapping of neuronal circuits. Using confocal microscopy, we have imaged the cellular-level expression of 109 transgenic lines in live 6 day post fertilization larvae, including 80 Gal4 enhancer trap lines, 9 Cre enhancer trap lines and 20 transgenic lines that express fluorescent proteins in defined gene-specific patterns. Image stacks were acquired at single micron resolution, together with a broadly expressed neural marker, which we used to align enhancer trap reporter patterns into a common 3-dimensional reference space. To facilitate use of this resource, we have written software that enables searching for transgenic lines that label cells within a selectable 3-dimensional region of interest (ROI) or neuroanatomical area. This software also enables the intersectional expression of transgenes to be predicted, a feature which we validated by detecting cells with co-expression of Cre and Gal4. Many of the imaged enhancer trap lines show intrinsic brain-specific expression. However, to increase the utility of lines that also drive expression in non-neuronal tissue we have designed a novel UAS reporter, that suppresses expression in heart, muscle, and skin through the incorporation of microRNA binding sites in a synthetic 3? untranslated region. Finally, we mapped the site of transgene integration, thus providing molecular identification of the expression pattern for most lines. Cumulatively, this library of enhancer trap lines provides genetic access to 70% of the larval brain and is therefore a powerful and broadly accessible tool for the dissection of neural circuits in larval zebrafish. © 2015 Marquart, Tabor, Brown, Strykowski, Varshney, LaFave, Mueller, Burgess, Higashijima and Burgess.
dc.relation.uri https://doi.org/10.3389/fncir.2015.00078
dc.rights Attribution 4.0 International (CC BY 4.0)
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject 3D Registration
dc.subject Cre
dc.subject Gal4
dc.subject Microrna
dc.subject Transgenic
dc.subject Zebrafish
dc.title A 3D searchable database of transgenic zebrafish gal4 and cre lines for functional neuroanatomy studies
dc.type Article
dc.date.published 2015
dc.citation.doi 10.3389/fncir.2015.00078
dc.citation.epage 17
dc.citation.issn 1662-5110
dc.citation.issue November
dc.citation.jtitle Frontiers in Neural Circuits
dc.citation.spage 1
dc.citation.volume 9
dc.contributor.authoreid muellert


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