Genomic targeting and mapping of agronomically important genes in wheat

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dc.contributor.author Kuraparthy, Vasu
dc.date.accessioned 2007-05-04T19:27:38Z
dc.date.available 2007-05-04T19:27:38Z
dc.date.issued 2007-05-04T19:27:38Z
dc.identifier.uri http://hdl.handle.net/2097/311
dc.description.abstract The wild relatives of crop plants are sources of useful genes, but such genes when transferred to agricultural crops are often associated with deleterious traits. Because most of the recombination and the disease resistance genes are localized towards the ends of wheat chromosomes, cryptic terminal alien segments, carrying rust resistance genes, were transferred from Aegilops geniculata (UgMg) and Ae. triuncialis (UtCt) into common wheat without the usual linkage drag. The alien segment with the leaf rust and stripe rust resistance genes Lr57 and Yr40 in translocation T5DL•5DS-5MgS(0.95) was found to be less than 3.3 cM in genetic length and spans less than four overlapping BAC/PAC clones of the syntenic rice chromosome arm 12L. The alien segment with leaf rust resistance gene Lr58, transferred from Ae. triuncialis, was found to be less than 5% of the chromosome arm 2BL of wheat in T2BS•2BL-2tL(0.95), further suggesting that it is feasible to transfer small alien segments with disease resistance genes. Resistance genes Lr57, Yr40 and Lr58 were transferred to Kansas hard red winter wheat cultivars by backcrossing and marker assisted selection. Tillering, a key component of grain yield, and seed color which influences seed dormancy and pre-harvest sprouting in wheat, are agronomically important domestication traits in wheat. A tiller inhibition mutant with monoculm phenotype was isolated and the mutated gene (tin3) was mapped on the distal region of chromosome arm 3AmL of T. monococcum. As a first step towards isolating candidate gene(s), the tin3 and the seed color gene (R-A1) of chromosome 3A were mapped in relation to physically mapped ESTs and STS markers developed based on synteny with rice. Physically mapped wheat ESTs provided a useful framework to identify closely related rice sequences and to establish the most likely syntenous region in rice for the wheat tin3 and R-A1 region. Comparative genomic analysis of the tin3 and R-A1 genomic regions with the corresponding region in rice localized the tin3 gene to a 324 kb region spanned by two overlapping BACs and the R-A1 gene was mapped to a single BAC of the colinear rice chromosome arm 1L. en
dc.description.sponsorship National Science Foundation; USDA; Kansas Wheat Commission en
dc.language.iso en_US en
dc.publisher Kansas State University en
dc.subject wheat and Aegilops en
dc.subject Rust resistance en
dc.subject Introgression en
dc.subject Germplasm en
dc.subject Tillering en
dc.subject Seed color en
dc.title Genomic targeting and mapping of agronomically important genes in wheat en
dc.type Dissertation en
dc.description.degree Doctor of Philosophy en
dc.description.level Doctoral en
dc.description.department Department of Plant Pathology en
dc.description.advisor Bikram S. Gill en
dc.subject.umi Agriculture, Agronomy (0285) en
dc.subject.umi Agriculture, Plant Pathology (0480) en
dc.subject.umi Biology, Genetics (0369) en
dc.date.published 2007 en
dc.date.graduationmonth May en


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