Solute Concentrations Influence Microbial Methanogenesis in Coal-bearing Strata of the Cherokee Basin, USA

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Show simple item record Kirk, Matthew F. en_US Wilson, Brien H. en_US Marquart, Kyle A. en_US Zeglin, Lydia H. en_US Vinson, David S. en_US Flynn, Theodore M. en_US 2016-01-19T20:39:02Z 2016-01-19T20:39:02Z
dc.description Microorganisms have contributed significantly to subsurface energy resources by converting organic matter in hydrocarbon reservoirs into methane, the main component of natural gas. In this study, we consider environmental controls on microbial populations in coal-bearing strata of the Cherokee basin, an unconventional natural gas resource in southeast Kansas, USA. Pennsylvanian-age strata in the basin contain numerous thin (0.4-1.1 m) coalbeds with marginal thermal maturities (0.5-0.7% Ro) that are interbedded with shale and sandstone. We collected gas, water, and microbe samples from 16 commercial coalbed methane wells for geochemical and microbiological analysis. The water samples were Na-Cl type with total dissolved solids (TDS) content ranging from 34.9 to 91.3 g L?1. Gas dryness values [C1/(C2 + C3)] averaged 2640 and carbon and hydrogen isotope ratios of methane differed from those of carbon dioxide and water, respectively, by an average of 65 and 183%. These values are thought to be consistent with gas that formed primarily by hydrogenotrophic methanogenesis. Results from cultivation assays and taxonomic analysis of 16S rRNA genes agree with the geochemical results. Cultivable methanogens were present in every sample tested, methanogen sequences dominate the archaeal community in each sample (avg 91%), and few archaeal sequences (avg 4.2%) were classified within Methanosarcinales, an order of methanogens known to contain methylotrophic methanogens. Although hydrogenotrophs appear dominant, geochemical and microbial analyses both indicate that the proportion of methane generated by acetoclastic methanogens increases with the solute content of formation water, a trend that is contrary to existing conceptual models. Consistent with this trend, beta diversity analyses show that archaeal diversity significantly correlates with formation water solute content. In contrast, bacterial diversity more strongly correlates with location than solute content, possibly as a result of spatial variation in the thermal maturity of the coalbeds. en_US
dc.description Citation: Kirk MF, Wilson BH, Marquart KA, Zeglin LH, Vinson DS and Flynn TM (2015) Solute Concentrations Influence Microbial Methanogenesis in Coal-bearing Strata of the Cherokee Basin, USA. Front. Microbiol. 6:1287. doi: 10.3389/fmicb.2015.01287 en_US
dc.relation.uri en_US
dc.rights Attribution 4.0 International (CC BY 4.0) en_US
dc.rights.uri en_US
dc.subject natural gas en_US
dc.subject unconventional reservoir en_US
dc.subject Cherokee basin en_US
dc.subject acetoclastic methanogenesis en_US
dc.subject hydrogenotrophic methanogenesis en_US
dc.title Solute Concentrations Influence Microbial Methanogenesis in Coal-bearing Strata of the Cherokee Basin, USA en_US
dc.type Text en_US 2015 en_US
dc.citation.doi 10.3389/fmicb.2016.00073 en_US
dc.citation.jtitle frontiers in Microbiology en_US
dc.citation.volume 6 en_US
dc.contributor.authoreid mfkirk en_US
dc.contributor.authoreid lzeglin en_US

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