Resistance of Kansas Sclerotinia homoeocarpa isolates to thiophanate-methyl and determination of associated β-tubulin mutation

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dc.contributor.author Ostrander, Jesse C.
dc.contributor.author Todd, Richard B.
dc.contributor.author Kennelly, Megan M.
dc.date.accessioned 2015-05-01T18:54:39Z
dc.date.available 2015-05-01T18:54:39Z
dc.date.issued 2014-06-06
dc.identifier.uri http://hdl.handle.net/2097/19174
dc.description Citation: Ostrander, J., Todd, R., & Kennelly, M. (2014). Resistance of Kansas Sclerotinia homoeocarpa Isolates to Thiophanate-Methyl and Determination of Associated β-Tubulin Mutation. Plant Health Progress, 15(2), 23-27. https://doi.org/10.1094/PHP-RS-13-0120.
dc.description.abstract Eighty-two isolates of Sclerotinia homoeocarpa from 12 sites in Kansas were evaluated for in vitro sensitivity to the methyl benzimidazole carbamate (MBC) fungicide thiophanate-methyl at the discriminatory dose of 10 μg/ml. Seventeen isolates were sensitive to thiophanate-methyl and the remaining isolates were resistant. Of the 65 isolates from golf course putting greens, two isolates were sensitive and the remaining 63 isolates were resistant. Six resistant and five sensitive isolates were also evaluated in greenhouse assays on fungicide-treated plants. The isolates that were sensitive to thiophanate-methyl in vitro did not cause any disease on thiophanate-methyl-treated plants, and those that were resistant in vitro caused blighting on treated plants equivalent to the nontreated controls. The entire β-tubulin gene was sequenced for four resistant and four sensitive isolates. The resistant isolates all harbored a substitution of alanine for glutamic acid at codon 198 (E198A). These results provide a starting point for further surveys and monitoring of fungicide sensitivity. en_US
dc.language.iso en_US en_US
dc.relation.uri https://doi.org/10.1094/PHP-RS-13-0120. en_US
dc.rights This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.rights.uri https://rightsstatements.org/page/InC/1.0/?language=en
dc.title Resistance of Kansas Sclerotinia homoeocarpa isolates to thiophanate-methyl and determination of associated β-tubulin mutation en_US
dc.type Text en_US
dc.date.published 2014 en_US
dc.citation.doi 10.1094/PHP-RS-13-0120 en_US
dc.citation.epage 27 en_US
dc.citation.issn 1535-1025
dc.citation.issue 2 en_US
dc.citation.jtitle Plant Health Progress en_US
dc.citation.spage 23 en_US
dc.citation.volume 15 en_US
dc.citation Ostrander, J., Todd, R., & Kennelly, M. (2014). Resistance of Kansas Sclerotinia homoeocarpa Isolates to Thiophanate-Methyl and Determination of Associated β-Tubulin Mutation. Plant Health Progress, 15(2), 23-27. https://doi.org/10.1094/PHP-RS-13-0120.
dc.contributor.authoreid rbtodd en_US
dc.contributor.authoreid kennelly en_US
dc.description.version Article: Version of Record


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