Development of a microfluidic flow cytometry platform with fluorescence and light scattering detection for the rapid characterization of circulating tumor cells

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dc.contributor.author Stewart-James, Samantha Ann
dc.date.accessioned 2015-04-24T18:36:36Z
dc.date.available 2015-04-24T18:36:36Z
dc.date.issued 2015-04-24
dc.identifier.uri http://hdl.handle.net/2097/19078
dc.description.abstract Circulating tumor cells (CTCs) have become a key component in the identification and treatment of cancer. Once dislodged from the main tumor, CTCs travel through the bloodstream and cause metastasis. Early detection and identification of these cells can help in the evaluation and prognosis of various types of cancer, as well as assisting in patient treatments by determining the spread of the disease. Here, a high-throughput microfluidic analysis technique is described that can efficiently detect and identify cells, with the specific identification of CTCs as a future application through fluorescent labeling in mind. As proof of principle, the device has been shown to detect and characterize individual human Jurkat (T-lymphocyte) cells at a rate of 100 cells/minute. The device employs micro-scale flow focusing to isolate individual cells. The cells are detected using both light scattering and laser-induced fluorescence to evaluate cell size and surface functionality. en_US
dc.description.sponsorship National Science Foundation GK-12 Graduate Training Fellowship Program. en_US
dc.language.iso en_US en_US
dc.publisher Kansas State University en
dc.subject Microfluidics en_US
dc.subject Flow cytometry en_US
dc.subject Single rare cell en_US
dc.subject Circulating tumor cell en_US
dc.subject Method development en_US
dc.subject Single-point detection en_US
dc.title Development of a microfluidic flow cytometry platform with fluorescence and light scattering detection for the rapid characterization of circulating tumor cells en_US
dc.type Thesis en_US
dc.description.degree Master of Science en_US
dc.description.level Masters en_US
dc.description.department Department of Chemistry en_US
dc.description.advisor Christopher T. Culbertson en_US
dc.subject.umi Chemistry (0485) en_US
dc.date.published 2015 en_US
dc.date.graduationmonth May en_US


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