Caudal regulates the spatiotemporal dynamics of pair-rule waves in Tribolium

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Show simple item record El-Sherif, Ezzat Zhu, Xin Fu, Jinping Brown, Susan J. 2015-04-07T16:06:17Z 2015-04-07T16:06:17Z 2015-04-07
dc.description.abstract In the short-germ beetle Tribolium castaneum, waves of pair-rule gene expression propagate from the posterior end of the embryo towards the anterior and eventually freeze into stable stripes, partitioning the anterior-posterior axis into segments. Similar waves in vertebrates are assumed to arise due to the modulation of a molecular clock by a posterior-to-anterior frequency gradient. However, neither a molecular candidate nor a functional role has been identified to date for such a frequency gradient, either in vertebrates or elsewhere. Here we provide evidence that the posterior gradient of Tc-caudal expression regulates the oscillation frequency of pair-rule gene expression in Tribolium. We show this by analyzing the spatiotemporal dynamics of Tc-even-skipped expression in strong and mild knockdown of Tc-caudal, and by correlating the extension, level and slope of the Tc-caudal expression gradient to the spatiotemporal dynamics of Tc-even-skipped expression in wild type as well as in different RNAi knockdowns of Tc-caudal regulators. Further, we show that besides its absolute importance for stripe generation in the static phase of the Tribolium blastoderm, a frequency gradient might serve as a buffer against noise during axis elongation phase in Tribolium as well as vertebrates. Our results highlight the role of frequency gradients in pattern formation. en_US
dc.language.iso en_US en_US
dc.relation.uri en_US
dc.subject Tribolium castaneum en_US
dc.subject Pair-rule waves en_US
dc.title Caudal regulates the spatiotemporal dynamics of pair-rule waves in Tribolium en_US
dc.type Article (publisher version) en_US 2014 en_US
dc.citation.doi doi:10.1371/journal.pgen.1004677 en_US
dc.citation.issue 10 en_US
dc.citation.jtitle PLoS Genetics en_US
dc.citation.spage e1004677 en_US
dc.citation.volume 10 en_US
dc.contributor.authoreid jinping en_US
dc.contributor.authoreid sjbrown en_US

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