A multicopper oxidase-related protein is essential for insect viability, longevity and ovary development

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dc.contributor.author Peng, Zeyu
dc.contributor.author Green, Peter G.
dc.contributor.author Arakane, Yasuyuki
dc.contributor.author Kanost, Michael R.
dc.contributor.author Gorman, Maureen J.
dc.date.accessioned 2015-03-23T20:32:06Z
dc.date.available 2015-03-23T20:32:06Z
dc.date.issued 2014-10-20
dc.identifier.uri http://hdl.handle.net/2097/18891
dc.description Citation: Peng Z, Green PG, Arakane Y, Kanost MR, Gorman MJ (2014) A Multicopper Oxidase-Related Protein Is Essential for Insect Viability, Longevity and Ovary Development. PLOS ONE 9(10): e111344. https://doi.org/10.1371/journal.pone.0111344
dc.description.abstract Typical multicopper oxidases (MCOs) have ten conserved histidines and one conserved cysteine that coordinate four copper atoms. These copper ions are required for oxidase activity. During our studies of insect MCOs, we discovered a gene that we named multicopper oxidase-related protein (MCORP). MCORPs share sequence similarity with MCOs, but lack many of the copper-coordinating residues. We identified MCORP orthologs in many insect species, but not in other invertebrates or vertebrates. We predicted that MCORPs would lack oxidase activity due to the absence of copper-coordinating residues. To test this prediction, we purified recombinant Tribolium castaneum (red flour beetle) MCORP and analyzed its enzymatic activity using a variety of substrates. As expected, no oxidase activity was detected. To study MCORP function in vivo, we analyzed expression profiles of TcMCORP and Anopheles gambiae (African malaria mosquito) MCORP, and assessed RNAi-mediated knockdown phenotypes. We found that both MCORPs are constitutively expressed at a low level in all of the tissues we analyzed. Injection of TcMCORP dsRNA into larvae resulted in 100% mortality prior to adult eclosion, with death occurring mainly during the pharate pupal stage or late pharate adult stage. Injection of TcMCORP dsRNA into pharate pupae resulted in the death of approximately 20% of the treated insects during the pupal to adult transition and a greatly shortened life span for the remaining insects. In addition, knockdown of TcMCORP in females prevented oocyte maturation and, thus, greatly decreased the number of eggs laid. These results indicate that TcMCORP is an essential gene and that its function is required for reproduction. An understanding of the role MCORP plays in insect physiology may help to develop new strategies for controlling insect pests. en_US
dc.language.iso en_US en_US
dc.relation.uri www.doi.org/10.1371/journal.pone.0111344 en_US
dc.rights This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.rights.uri https://plos.org/open-science/why-open-access/
dc.rights.uri https://rightsstatements.org/page/InC/1.0/?language=en
dc.subject Multicopper oxidases en_US
dc.subject Tribolium castaneum en_US
dc.subject Anopheles gambiae en_US
dc.title A multicopper oxidase-related protein is essential for insect viability, longevity and ovary development en_US
dc.type Article (publisher version) en_US
dc.date.published 2014 en_US
dc.citation.doi 10.1371/journal.pone.0111344 en_US
dc.citation.issue 10 en_US
dc.citation.jtitle PLoS ONE en_US
dc.citation.spage e111344 en_US
dc.citation.volume 9 en_US
dc.citation Peng Z, Green PG, Arakane Y, Kanost MR, Gorman MJ (2014) A Multicopper Oxidase-Related Protein Is Essential for Insect Viability, Longevity and Ovary Development. PLOS ONE 9(10): e111344. https://doi.org/10.1371/journal.pone.0111344
dc.contributor.authoreid zeyupeng en_US
dc.contributor.authoreid kanost en_US
dc.contributor.authoreid mgorman en_US


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