Use of PCR to identify Leptospira in kidneys of big brown bats (Eptesicus fuscus) in Kansas and Nebraska, USA

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dc.contributor.author Harkin, Kenneth R.
dc.contributor.author Hays, Michael P.
dc.contributor.author Davis, Rolan D.
dc.contributor.author Moore, Michael C.
dc.date.accessioned 2014-11-04T20:44:05Z
dc.date.available 2014-11-04T20:44:05Z
dc.date.issued 2014-11-04
dc.identifier.uri http://hdl.handle.net/2097/18637
dc.description.abstract Bats have been implicated as potential carriers of Leptospira as a result of surveys, mostly in Australia and South America. We measured the prevalence of pathogenic leptospires in kidneys of bats from Kansas and Nebraska. From 7 August 2012 to 21 August 2012, we extracted DNA from kidneys of 98 big brown bats (Eptesicus fuscus) submitted and found negative for rabies. The DNA was processed in a two-step, seminested PCR assay with a dual-labeled Taqman probe specific for pathogenic leptospires. As a negative control, we used a saprophytic leptospire (Leptospira biflexa Patoc) and, as a pathogenic control, Leptospira interrogans Canicola. All bat kidneys were negative for pathogenic leptospires, suggesting that it is unlikely that the big brown bat, one of the most prevalent bat species in North America, is a reservoir for transmission of leptospires to dogs or humans. en_US
dc.language.iso en_US en_US
dc.relation.uri http://www.jwildlifedis.org/doi/abs/10.7589/2013-08-201 en_US
dc.subject Big brown bat en_US
dc.subject Eptesicus fuscus en_US
dc.subject Leptospirosis en_US
dc.subject PCR en_US
dc.title Use of PCR to identify Leptospira in kidneys of big brown bats (Eptesicus fuscus) in Kansas and Nebraska, USA en_US
dc.type Article (publisher version) en_US
dc.date.published 2014 en_US
dc.citation.doi doi:10.7589/2013-08-201 en_US
dc.citation.epage 654 en_US
dc.citation.issue 3 en_US
dc.citation.jtitle Journal of Wildlife Diseases en_US
dc.citation.spage 651 en_US
dc.citation.volume 50 en_US
dc.contributor.authoreid harkin en_US
dc.contributor.authoreid mhays en_US
dc.contributor.authoreid rdd8889 en_US
dc.contributor.authoreid mcmoore en_US


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