Association analysis of stem rust resistance in U.S. winter wheat

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dc.contributor.author Zhang, Dadong
dc.contributor.author Bowden, Robert L.
dc.contributor.author Yu, Jianming
dc.contributor.author Carver, Brett F.
dc.contributor.author Bai, Guihua
dc.date.accessioned 2014-10-01T18:39:46Z
dc.date.available 2014-10-01T18:39:46Z
dc.date.issued 2014-10-01
dc.identifier.uri http://hdl.handle.net/2097/18357
dc.description.abstract Stem rust has become a renewed threat to global wheat production after the emergence and spread of race TTKSK (also known as Ug99) and related races from Africa. To elucidate U.S. winter wheat resistance genes to stem rust, association mapping was conducted using a panel of 137 lines from cooperative U.S. winter wheat nurseries from 2008 and simple sequence repeat (SSR) and sequence tagged site (STS) markers across the wheat genome. Seedling infection types were evaluated in a greenhouse experiment using six U.S. stem rust races (QFCSC, QTHJC, RCRSC, RKQQC, TPMKC and TTTTF) and TTKSK, and adult plant responses to bulked U.S. races were evaluated in a field experiment. A linearization algorithm was used to convert the qualitative Stakman scale seedling infection types for quantitative analysis. Association mapping successfully detected six known stem rust seedling resistance genes in U.S. winter wheat lines with frequencies: Sr6 (12%), Sr24 (9%), Sr31 (15%), Sr36 (9%), Sr38 (19%), and Sr1RS[superscript Amigo] (8%). Adult plant resistance gene Sr2 was present in 4% of lines. SrTmp was postulated to be present in several hard winter wheat lines, but the frequency could not be accurately determined. Sr38 was the most prevalent Sr gene in both hard and soft winter wheat and was the most effective Sr gene in the adult plant field test. Resistance to TTKSK was associated with nine markers on chromosome 2B that were in linkage disequilibrium and all of the resistance was attributed to the Triticum timopheevii chromosome segment carrying Sr36. Potential novel rust resistance alleles were associated with markers Xwmc326-203 on 3BL, Xgwm160-195 and Xwmc313-225 on 4AL near Sr7, Xgwm495-182 on 4BL, Xwmc622-147 and Xgwm624-146 on 4DL, and Xgwm334-123 on 6AS near Sr8. Xwmc326-203 was associated with adult plant resistance to bulked U.S. races and Xgwm495-182 was associated with seedling resistance to TTKSK. en_US
dc.language.iso en_US en_US
dc.relation.uri http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0103747 en_US
dc.subject Stem rust en_US
dc.subject Winter wheat en_US
dc.subject U.S. winter wheat en_US
dc.subject Resistance genes en_US
dc.title Association analysis of stem rust resistance in U.S. winter wheat en_US
dc.type Article (publisher version) en_US
dc.date.published 2014 en_US
dc.citation.doi doi:10.1371/journal.pone.0103747 en_US
dc.citation.issue 7 en_US
dc.citation.jtitle PLoS ONE en_US
dc.citation.spage e103747 en_US
dc.citation.volume 9 en_US
dc.contributor.authoreid rbowden en_US
dc.contributor.authoreid gbai en_US


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