Molecular markers for species identification of Hessian fly males caught on sticky pheromone traps

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Show simple item record Chen, Ming-Shun Wheeler, Shanda Davis, Holly Whitworth, R. Jeffery Knutson, Allen Giles, Kristopher L. Royer, Tom A. Skinner, Margaret 2014-08-20T20:49:43Z 2014-08-20T20:49:43Z 2014-08-20
dc.description.abstract Pheromone traps have been widely used to monitor insect population activity. However, sticky pheromone traps for the Hessian fly (Mayetiola destructor), one of the most destructive pests of wheat, have been used only in recent years. Hessian fly male adults are small and fragile, and preserving specimens during sorting of sticky pheromone traps is a challenge when intact specimens are often required to visually distinguish them from related insects such as fungus gnats. In this study, we have established a quick and reliable method based on polymerase chain reaction markers to correctly distinguish Hessian fly males from other closely related insects. Two Hessian fly-specific markers were established, one based on the trypsin gene MDP-10 and the other based on a gene encoding the salivary gland protein SSGP31‐5. Both markers provided >98% identification success of 110 Hessian fly samples prepared from single insects. The method should provide a useful tool to allow for identification of Hessian fly individuals on sticky pheromone traps or in other situations when Hessian fly eggs, larvae, pupae, and adults are difficult to distinguish from other insects. en_US
dc.language.iso en_US en_US
dc.relation.uri en_US
dc.rights Permission to archive granted by Entomological Society of America, July 17, 2014. This article is the copyright property of the Entomological Society of America and may not be used for any commercial or other private purpose without specific written permission of the Entomological Society of America. en_US
dc.subject Mayetiola destructor en_US
dc.subject Hessian fly en_US
dc.subject Pheromone trap en_US
dc.subject Wheat en_US
dc.subject Molecular marker en_US
dc.title Molecular markers for species identification of Hessian fly males caught on sticky pheromone traps en_US
dc.type Article (publisher version) en_US 2014 en_US
dc.citation.doi doi:10.1603/EC13384 en_US
dc.citation.epage 1117 en_US
dc.citation.issue 3 en_US
dc.citation.jtitle Journal of Economic Entomology en_US
dc.citation.spage 1110 en_US
dc.citation.volume 107 en_US
dc.contributor.authoreid mchen en_US
dc.contributor.authoreid jwhitwor en_US

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