Evolutionary characterization of pig interferon-inducible transmembrane gene family and member expression dynamics in tracheobronchial lymph nodes of pigs infected with swine respiratory disease viruses

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dc.contributor.author Miller, Laura C.
dc.contributor.author Jiang, Zhihua
dc.contributor.author Sang, Yongming
dc.contributor.author Harhay, Gregory P.
dc.contributor.author Lager, Kelly M.
dc.date.accessioned 2014-07-16T21:45:37Z
dc.date.available 2014-07-16T21:45:37Z
dc.date.issued 2014-07-16
dc.identifier.uri http://hdl.handle.net/2097/17925
dc.description.abstract Studies have found that a cluster of duplicated gene loci encoding the interferon-inducible transmembrane proteins (IFITMs) family have antiviral activity against several viruses, including influenza A virus. The gene family has 5 and 7 members in humans and mice, respectively. Here, we confirm the current annotation of pig IFITM1, IFITM2, IFITM3, IFITM5, IFITM1L1 and IFITM1L4, manually annotated IFITM1L2, IFITM1L3, IFITM5L, IFITM3L1 and IFITM3L2, and provide expressed sequence tag (EST) and/or mRNA evidence, not contained with the NCBI Reference Sequence database (RefSeq), for the existence of IFITM6, IFITM7 and a new IFITM1-like (IFITM1LN) gene in pigs. Phylogenic analyses showed seven porcine IFITM genes with highly conserved human/mouse orthologs known to have anti-viral activity. Digital Gene Expression Tag Profiling (DGETP) of swine tracheobronchial lymph nodes (TBLN) of pigs infected with swine influenza virus (SIV), porcine pseudorabies virus, porcine reproductive and respiratory syndrome virus or porcine circovirus type 2 over 14 days post-inoculation (dpi) showed that gene expression abundance differs dramatically among pig IFITM family members, ranging from 0 to over 3,000 tags per million. In particular, SIV upregulated IFITM1 by 5.9 fold at 3 dpi. Bayesian framework further identified pig IFITM1 and IFITM3 as differentially expressed genes in the overall transcriptome analysis. In addition to being a component of protein complexes involved in homotypic adhesion, the IFITM1 is also associated with pathways related to regulation of cell proliferation and IFITM3 is involved in immune responses. en_US
dc.language.iso en_US en_US
dc.relation.uri http://www.sciencedirect.com/science/article/pii/S0165242714000518 en_US
dc.subject Interferon-inducible transmembrane protein family member en_US
dc.subject IFITM en_US
dc.subject Swine genome en_US
dc.subject Influenza virus en_US
dc.title Evolutionary characterization of pig interferon-inducible transmembrane gene family and member expression dynamics in tracheobronchial lymph nodes of pigs infected with swine respiratory disease viruses en_US
dc.type Article (author version) en_US
dc.date.published 2014 en_US
dc.citation.doi doi:10.1016/j.vetimm.2014.02.015 en_US
dc.citation.epage 191 en_US
dc.citation.issue 3-4 en_US
dc.citation.jtitle Veterinary Immunology and Immunopathology en_US
dc.citation.spage 180 en_US
dc.citation.volume 159 en_US
dc.contributor.authoreid ysang en_US


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