North American triple reassortant and Eurasian H1N1 swine influenza viruses do not readily reassort to generate a 2009 pandemic H1N1-like virus

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dc.contributor.author Ma, Wenjun
dc.contributor.author Liu, Qinfang
dc.contributor.author Qiao, Chuanling
dc.contributor.author del Real, Gustavo
dc.contributor.author García-Sastre, Adolfo
dc.contributor.author Webby, Richard J.
dc.contributor.author Richt, Juergen A.
dc.date.accessioned 2014-06-17T20:49:54Z
dc.date.available 2014-06-17T20:49:54Z
dc.date.issued 2014-06-17
dc.date.issued 2014-03-11
dc.identifier.uri http://hdl.handle.net/2097/17858
dc.description.abstract The 2009 pandemic H1N1 virus (pH1N1) was derived through reassortment of North American triple reassortant and Eurasian avian-like swine influenza viruses (SIVs). To date, when, how and where the pH1N1 arose is not understood. To investigate viral reassortment, we coinfected cell cultures and a group of pigs with or without preexisting immunity with a Eurasian H1N1 virus, A/Swine/Spain/53207/2004 (SP04), and a North American triple reassortant H1N1 virus, A/Swine/Kansas/77778/2007 (KS07). The infected pigs were cohoused with one or two groups of contact animals to investigate viral transmission. In coinfected MDCK or PK15 continuous cell lines with KS07 and SP04 viruses, more than 20 different reassortant viruses were found. In pigs without or with preexisting immunity (immunized with commercial inactivated swine influenza vaccines) and coinfected with both viruses, six or seven reassortant viruses, as well as the parental viruses, were identified in bronchoalveolar lavage fluid samples from the lungs. Interestingly, only one or two viruses transmitted to and were detected in contact animals. No reassortant containing a gene constellation similar to that of pH1N1 virus was found in either coinfected cells or pigs, indicating that the reassortment event that resulted in the generation of this virus is a rare event that likely involved specific viral strains and/or a favorable, not-yet-understood environment. en_US
dc.language.iso en_US en_US
dc.relation.uri http://doi.org/10.1128/mBio.00919-13 en_US
dc.rights This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited. en_US
dc.subject H1N1 en_US
dc.subject Avian-like swine influenza viruses en_US
dc.subject Swine influenza viruses en_US
dc.subject Influenza en_US
dc.title North American triple reassortant and Eurasian H1N1 swine influenza viruses do not readily reassort to generate a 2009 pandemic H1N1-like virus en_US
dc.type Article (publisher version) en_US
dc.date.published 2014 en_US
dc.citation.doi 10.1128/mBio.00919-13 en_US
dc.citation.issue 2 en_US
dc.citation.jtitle mBio en_US
dc.citation.spage e00919-13 en_US
dc.citation.volume 5 en_US
dc.contributor.authoreid wjma en_US
dc.contributor.authoreid qinfangliu en_US
dc.contributor.authoreid jricht en_US


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