Production of Autopolyploid Lowland Switchgrass Lines Through In Vitro Chromosome Doubling.

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dc.contributor.author Yang, Zhiyong
dc.contributor.author Shen, Zhengxing
dc.contributor.author Tetreault, Hannah
dc.contributor.author Johnson, Loretta
dc.contributor.author Friebe, Bernd
dc.contributor.author Frazier, Taylor
dc.contributor.author Huang, Lin-kai
dc.contributor.author Burklew, Caitlin
dc.contributor.author Zhang, Xin-Quan
dc.contributor.author Zhao, Bingyu
dc.date.accessioned 2014-05-09T16:46:16Z
dc.date.available 2014-05-09T16:46:16Z
dc.date.issued 2014-05-09
dc.identifier.uri http://hdl.handle.net/2097/17734
dc.description.abstract Switchgrass is considered one of the most promising energy crops. However, breeding of elite switchgrass cultivars is required to meet the challenges of large scale and sustainable biomass production. As a native perennial adapted to North America, switchgrass has lowland and upland ecotypes, where most lowland ecotypes are tetraploid (2n=4x=36), and most upland ecotypes are predominantly octoploid (2n=8x=72). Hybridization between lowland and upland switchgrass plants could identify new cultivars with heterosis. However, crossing between tetraploid and octoploid switchgrass is rare in nature. Therefore, in order to break down the cross incompatibility barrier between tetraploid lowland and octoploid upland switchgrass lines, we developed autoployploid switchgrass lines from an anueploid lowland cv. Alamo. In this study, colchicine was used in liquid and solid mediums to chemically induce chromosome doubling in embryogenic calli derived from cv. Alamo. Thirteen autopolyploid switchgrass lines were regenerated from seedlings and identified using flow cytometry. The autoplyploid switchgrass plants exhibited increased stomata aperture and stem size in comparison with the cv. Alamo. The most autooplyploid plants were regenerated from switchgrass calli that were treated with 0.04 % colchicine in liquid medium for 13 days. One autopolyploid switchgrass line, VT8-1, was successfully crossed to the octoploid upland cv. Blackwell. The autoployploid and the derived inter-ecotype hybrids were confirmed by in situ hybridization and molecular marker analysis. Therefore, the results of this study show that an autopolyploid, generated by chemically induced chromosome doubling of lowland cv. Alamo, is cross compatible with upland octoploid switchgrass cultivars. The outcome of this study may have significant applications in switchgrass hybrid breeding. en_US
dc.language.iso en_US en_US
dc.relation.uri http://link.springer.com/article/10.1007/s12155-013-9364-x en_US
dc.subject Bioenergy en_US
dc.subject Switchgrass en_US
dc.subject Autopolyploids en_US
dc.subject Chromosome doubling en_US
dc.subject Cytogenetics en_US
dc.subject Biofeedstocks en_US
dc.title Production of Autopolyploid Lowland Switchgrass Lines Through In Vitro Chromosome Doubling. en_US
dc.type Article (author version) en_US
dc.date.published 2014 en_US
dc.citation.doi 10.1007/s12155-013-9364-x en_US
dc.citation.epage 242 en_US
dc.citation.issue 1 en_US
dc.citation.jtitle Bioenergy Research en_US
dc.citation.spage 232 en_US
dc.citation.volume 7 en_US
dc.contributor.authoreid friebe en_US


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