Changes in gene expression in the larval gut of Ostrinia nubilalis in response to Bacillus thuringiensis Cry1Ab protoxin ingestion

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dc.contributor.author Yao, Jianxiu
dc.contributor.author Buschman, Lawrent L.
dc.contributor.author Lu, Nanyan
dc.contributor.author Khajuria, Chitvan
dc.contributor.author Zhu, Kun Yan
dc.date.accessioned 2014-05-09T15:09:06Z
dc.date.available 2014-05-09T15:09:06Z
dc.date.issued 2014-05-09
dc.identifier.uri http://hdl.handle.net/2097/17730
dc.description.abstract We developed a microarray based on 2895 unique transcripts assembled from 15,000 cDNA sequences from the European corn borer (Ostrinia nubilalis) larval gut. This microarray was used to monitor gene expression in early third-instar larvae of Bacillus thuringiensis (Bt)-susceptible O. nubilalis after 6 h feeding on diet, with or without the Bt Cry1Ab protoxin. We identified 174 transcripts, for which the expression was changed more than two-fold in the gut of the larvae fed Cry1Ab protoxin (p < 0.05), representing 80 down-regulated and 94 up-regulated transcripts. Among 174 differentially expressed transcripts, 13 transcripts putatively encode proteins that are potentially involved in Bt toxicity, and these transcripts include eight serine proteases, three aminopeptidases, one alkaline phosphatase, and one cadherin. The expressions of trypsin-like protease and three aminopeptidase transcripts were variable, but two potential Bt-binding proteins, alkaline phosphatase and cadherin were consistently up-regulated in larvae fed Cry1Ab protoxin. The significantly up and down-regulated transcripts may be involved in Cry1Ab toxicity by activation, degradation, toxin binding, and other related cellular responses. This study is a preliminary survey of Cry1Ab protoxin-induced transcriptional responses in O. nubilalis gut and our results are expected to help with further studies on Bt toxin-insect interactions at the molecular level. en_US
dc.language.iso en_US en_US
dc.relation.uri http://www.mdpi.com/2072-6651/6/4/1274 en_US
dc.subject Bacillus thuringiensis en_US
dc.subject European corn borer en_US
dc.subject Ostrinia nubilalis en_US
dc.subject Cry1Ab protoxin en_US
dc.subject Microarray en_US
dc.subject Transcriptional response en_US
dc.title Changes in gene expression in the larval gut of Ostrinia nubilalis in response to Bacillus thuringiensis Cry1Ab protoxin ingestion en_US
dc.type Article (publisher version) en_US
dc.date.published 2014 en_US
dc.citation.doi doi:10.3390/toxins6041274 en_US
dc.citation.epage 1294 en_US
dc.citation.issue 4 en_US
dc.citation.jtitle Toxins en_US
dc.citation.spage 1274 en_US
dc.citation.volume 6 en_US
dc.contributor.authoreid lbuschma en_US
dc.contributor.authoreid nanyan en_US
dc.contributor.authoreid kzhu en_US


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