Transcriptomics of induced defense responses to greenbug aphid feeding in near isogenic wheat lines.

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Show simple item record Reddy, Srirama Krishna Weng, Yiqun Rudd, Jackie C. Akhunova, Alina R. Liu, Shuyu 2014-05-08T13:22:17Z 2014-05-08T13:22:17Z 2014-05-08
dc.description.abstract The greenbug aphid, Schizaphis graminum (Rondani) is an important cereal pest, periodically threatening wheat yields in the United States and around the world. The single dominant gene, Gb3-based resistance is highly durable against prevailing greenbug biotypes under field conditions; however, the molecular mechanisms of Gb3-mediated defense responses remain unknown. We used Affymetrix GeneChip Wheat Genome Arrays to investigate the transcriptomics of host defense responses upon greenbug feeding on resistant and susceptible bulks (RB and SB, respectively) derived from two near-isogenic lines. The study identified 692 differentially expressed transcripts and further functional classification recognized 122 transcripts that are putatively associated to mediate biotic stress responses. In RB, Gb3-mediated resistance resulted in activation of transmembrane receptor kinases and signaling-related transcripts involved in early signal transduction cascades. While in SB, transcripts mediating final steps in jasmonic acid biosynthesis, redox homeostasis, peroxidases, glutathione S-transferases, and notable defense-related secondary metabolites were induced. Also transcripts involved in callose and cell wall decomposition were elevated SB, plausibly to facilitate uninterrupted feeding operations. These results suggest that Gb3-mediated resistance is less vulnerable to cell wall modification and the data provides ample tools for further investigations concerning R gene based model of resistance. en_US
dc.language.iso en_US en_US
dc.relation.uri en_US
dc.subject Aphid resistance en_US
dc.subject Greenbug en_US
dc.subject Jasmonic acid en_US
dc.subject Microarray en_US
dc.subject Transcriptomics en_US
dc.subject Wheat en_US
dc.title Transcriptomics of induced defense responses to greenbug aphid feeding in near isogenic wheat lines. en_US
dc.type Article (author version) en_US 2013 en_US
dc.citation.doi 10.1016/j.plantsci.2013.08.002 en_US
dc.citation.epage 36 en_US
dc.citation.jtitle Plant Science en_US
dc.citation.spage 26 en_US
dc.citation.volume 212 en_US
dc.contributor.authoreid akhunova en_US

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