Using transcription of six Puccinia triticina races to identify the effective secretome during infection of wheat

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dc.contributor.author Bruce, Myron
dc.contributor.author Neugebauer, Kerri A.
dc.contributor.author Joly, David L.
dc.contributor.author Migeon, Pierre
dc.contributor.author Cuomo, Christina A.
dc.contributor.author Wang, Shichen
dc.contributor.author Akhunov, Eduard D.
dc.contributor.author Bakkeren, Guus
dc.contributor.author Kolmer, James A.
dc.contributor.author Fellers, John P.
dc.date.accessioned 2014-04-30T16:25:40Z
dc.date.available 2014-04-30T16:25:40Z
dc.date.issued 2014-04-30
dc.date.issued 2014-01-13
dc.identifier.uri http://hdl.handle.net/2097/17672
dc.description Citation: Bruce, M., . . . & Fellers, J. (2014). Using transcription of six Puccinia triticina races to identify the effective secretome during infection of wheat. Frontiers in Plant Science, 4(1), 520. https://doi.org/10.3389/fpls.2013.00520
dc.description.abstract Wheat leaf rust, caused by the basidiomycete Puccinia triticina, can cause yield losses of up to 20% in wheat producing regions. During infection, the fungus forms haustoria that secrete proteins into the plant cell and effect changes in plant transcription, metabolism, and defense. It is hypothesized that new races emerge as a result of overcoming plant resistance via changes in the secreted effector proteins. To understand gene expression during infection and find genetic differences associated with races, RNA from wheat leaves infected with six different rust races, at 6 days post inoculation, was sequenced using Illumina. As P. triticina is an obligate biotroph, RNA from both the host and fungi were present and separated by alignment to the P. triticina genome and a wheat EST reference. A total of 222,571 rust contigs were assembled from 165 million reads. An examination of the resulting contigs revealed 532 predicted secreted proteins among the transcripts. Of these, 456 were found in all races. Fifteen genes were found with amino acid changes, corresponding to putative avirulence effectors potentially recognized by 11 different leaf rust resistance (Lr) genes. Twelve of the potential avirulence effectors have no homology to known genes. One gene had significant similarity to cerato-platanin, a known fungal elicitor, and another showed similarity to fungal tyrosinase, an enzyme involved in melanin synthesis. Temporal expression profiles were developed for these genes by qRT-PCR and show that the genes expression patterns were consistent between races from infection initiation to just prior to spore eruption. en_US
dc.language.iso en_US en_US
dc.relation.uri https://doi.org/10.3389/fpls.2013.00520 en_US
dc.rights © 2014 Bruce, Neugebauer, Joly, Migeon, Cuomo, Wang, Akhunov, Bakkeren, Kolmer and Fellers. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.rights.uri http://creativecommons.org/licenses/by/3.0/
dc.subject Puccinia triticina en_US
dc.subject Secreted peptides en_US
dc.subject Effectors en_US
dc.subject Leaf rust en_US
dc.subject RNA sequencing en_US
dc.title Using transcription of six Puccinia triticina races to identify the effective secretome during infection of wheat en_US
dc.type Text en_US
dc.date.published 2014 en_US
dc.citation.doi 10.3389/fpls.2013.00520 en_US
dc.citation.issn 1664-462X
dc.citation.issue 1 en_US
dc.citation.jtitle Frontiers in Plant Science en_US
dc.citation.spage 520 en_US
dc.citation.volume 4 en_US
dc.citation Bruce, M., . . . & Fellers, J. (2014). Using transcription of six Puccinia triticina races to identify the effective secretome during infection of wheat. Frontiers in Plant Science, 4(1), 520. https://doi.org/10.3389/fpls.2013.00520
dc.contributor.authoreid eakhunov en_US
dc.contributor.authoreid jfellers en_US
dc.description.version Article: Verision of Record


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© 2014 Bruce, Neugebauer, Joly, Migeon, Cuomo, Wang, Akhunov, Bakkeren, Kolmer and Fellers. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Except where otherwise noted, the use of this item is bound by the following: © 2014 Bruce, Neugebauer, Joly, Migeon, Cuomo, Wang, Akhunov, Bakkeren, Kolmer and Fellers. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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