A UV detector for microfluidic devices

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dc.contributor.author Weldegebriel, Amos
dc.date.accessioned 2014-04-28T16:49:09Z
dc.date.available 2014-04-28T16:49:09Z
dc.date.issued 2014-04-28
dc.identifier.uri http://hdl.handle.net/2097/17626
dc.description.abstract Chemical separation involves selective movement of a component out of a region shared by multiple components into a region where it is the major occupant. The history of the field of chemical separations as a concept can be dated back to ancient times when people started improving the quality of life by separation of good materials from bad ones. Since then the field of chemical separation has become one of the most continually evolving branches of chemical science and encompasses numerous different techniques and principles. An analytical chemist’s quest for a better way of selective identification and quantification of a component by separating it from its mixture is the cause for these ever evolving techniques. As a result, today there are numerous varieties of analytical techniques for the separation of complex mixtures. High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Capillary Electrophoresis (CE) and Gel Electrophoresis are a few out of a long list. Each these techniques manipulates the different physical and chemical properties of an analyte to achieve a useful separation and thus certain techniques will be suited for certain molecules. This work primarily focuses on the use of Capillary Electrophoresis as a separation technique. The mechanism of separation in Capillary Zone Electrophoresis and principles of UV detection will discussed in chapter one. Chapter two contains a discussion about the application of Capillary Electrophoresis (CE) on microfluidc devices. This will include sections on: microfabrication techniques of PDMS and photosensitized PDMS (photoPDMS), a UV detector for microfluidic devices and its application for the detection of wheat proteins. In Chapter three we report the experimental part of this project which includes; investigations on the effect of UV exposure time and thermal curing time on feature dimensions of photoPDMS microfluidic device, investigations on the injection and separation performances of the device, characterization of a UV detector set up and its application for the separation and detection of wheat gliadin proteins. The results of these investigations are presented in chapter four. en_US
dc.language.iso en_US en_US
dc.publisher Kansas State University en
dc.subject UV detector for microfluidic devices en_US
dc.subject Microchip capillary electrophoresis en_US
dc.subject Gliadin wheat protein en_US
dc.title A UV detector for microfluidic devices en_US
dc.type Thesis en_US
dc.description.degree Master of Science en_US
dc.description.level Masters en_US
dc.description.department Department of Chemistry en_US
dc.description.advisor Christopher T. Culbertson en_US
dc.subject.umi Chemistry (0485) en_US
dc.date.published 2014 en_US
dc.date.graduationmonth May en_US

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