Overexpression of IL-10 in C2D macrophages promotes a macrophage phenotypic switch in adipose tissue environments

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dc.contributor.author Xie, Linglin
dc.contributor.author Fu, Qiang
dc.contributor.author Ortega, Teresa M.
dc.contributor.author Zhou, Lun
dc.contributor.author Rasmussen, Dane
dc.contributor.author O’Keefe, Jacy
dc.contributor.author Zhang, Ke K.
dc.contributor.author Chapes, Stephen K.
dc.date.accessioned 2014-04-18T20:38:18Z
dc.date.available 2014-04-18T20:38:18Z
dc.date.issued 2014-04-18
dc.identifier.uri http://hdl.handle.net/2097/17332
dc.description.abstract Adipose tissue macrophages are a heterogeneous collection of classically activated (M1) and alternatively activated (M2) macrophages. Interleukin 10 (IL-10) is an anti-inflammatory cytokine, secreted by a variety of cell types including M2 macrophages. We generated a macrophage cell line stably overexpressing IL-10 (C2D-IL10) and analyzed the C2D-IL10 cells for several macrophage markers after exposure to adipocytes compared to C2D cells transfected with an empty vector (C2D-vector). C2D-IL10 macrophage cells expressed more CD206 when co-cultured with adipocytes than C2D-vector cells; while the co-cultured cell mixture also expressed higher levels of Il4, Il10, Il1β and Tnf. Since regular C2D cells traffic to adipose tissue after adoptive transfer, we explored the impact of constitutive IL-10 expression on C2D-IL10 macrophages in adipose tissue in vivo. Adipose tissue-isolated C2D-IL10 cells increased the percentage of CD206+, CD301+, CD11c−CD206+ (M2) and CD11c+CD206+ (M1b) on their cell surface, compared to isolated C2D-vector cells. These data suggest that the expression of IL-10 remains stable, alters the C2D-IL10 macrophage cell surface phenotype and may play a role in regulating macrophage interactions with the adipose tissue. en_US
dc.language.iso en_US en_US
dc.relation.uri http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0086541 en_US
dc.subject Macrophages en_US
dc.subject Adipose tissue en_US
dc.subject Interleukin 10 en_US
dc.title Overexpression of IL-10 in C2D macrophages promotes a macrophage phenotypic switch in adipose tissue environments en_US
dc.type Article (publisher version) en_US
dc.date.published 2014 en_US
dc.citation.doi doi:10.1371/journal.pone.0086541 en_US
dc.citation.issue 1 en_US
dc.citation.jtitle PLoS ONE en_US
dc.citation.spage e86541 en_US
dc.citation.volume 9 en_US
dc.contributor.authoreid skcbiol en_US


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