Development of a wheat single gene FISH map for analyzing homoeologous relationship and chromosomal rearrangements within the Triticeae

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dc.contributor.author Danilova, Tatiana V.
dc.contributor.author Friebe, Bernd R.
dc.contributor.author Gill, Bikram S.
dc.date.accessioned 2014-03-14T19:48:15Z
dc.date.available 2014-03-14T19:48:15Z
dc.date.issued 2014-03-14
dc.identifier.uri http://hdl.handle.net/2097/17229
dc.description Citation: Danilova, T., . . . & Gill, B. (2014). Development of a wheat single gene FISH map for analyzing homoeologous relationship and chromosomal rearrangements within the Triticeae. Theoretical and Applied Genetics, 127(3), 715-730. https://doi.org/10.1007/s00122-013-2253-z
dc.description.abstract To transfer agronomically important genes from wild relatives to bread wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) by induced homoeologous recombination, it is important to know the chromosomal relationships of the species involved. Fluorescence in situ hybridization (FISH) can be used to study chromosome structure. The genomes of allohexaploid bread wheat and other species from the Triticeae tribe are colinear to some extent, i.e., composed of homoeoloci at similar positions along the chromosomes, and with genic regions being highly conserved. To develop cytogenetic markers specific for genic regions of wheat homoeologs, we selected more than 60 full-length wheat cDNAs using BLAST against mapped expressed sequence tags and used them as FISH probes. Most probes produced signals on all three homoeologous chromosomes at the expected positions. We developed a wheat physical map with several cDNA markers located on each of the 14 homoeologous chromosome arms. The FISH markers confirmed chromosome rearrangements within wheat genomes and were successfully used to study chromosome structure and homoeology in wild Triticeae species. FISH analysis detected 1U-6U chromosome translocation in the genome of Aegilops umbellulata, showed colinearity between chromosome A of Ae. caudata and group-1 wheat chromosomes, and between chromosome arm 7S#3L of Thinopyrum intermedium and the long arm of the group-7 wheat chromosomes. en_US
dc.language.iso en_US en_US
dc.relation.uri https://doi.org/10.1007/s00122-013-2253-z en_US
dc.rights This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. en_US
dc.rights.uri https://creativecommons.org/licenses/by/3.0/us/
dc.rights.uri https://www.springer.com/gp/open-access/publication-policies/self-archiving-policy
dc.subject Wheat en_US
dc.subject Triticeae en_US
dc.subject Chromosomal rearrangements en_US
dc.subject Fluorescence in situ hybridization en_US
dc.title Development of a wheat single gene FISH map for analyzing homoeologous relationship and chromosomal rearrangements within the Triticeae en_US
dc.type Text en_US
dc.date.published 2014 en_US
dc.citation.doi 10.1007/s00122-013-2253-z en_US
dc.citation.epage 730 en_US
dc.citation.issn 0040-5752
dc.citation.issue 3 en_US
dc.citation.jtitle Theoretical and Applied Genetics en_US
dc.citation.spage 715 en_US
dc.citation.volume 127 en_US
dc.citation Danilova, T., . . . & Gill, B. (2014). Development of a wheat single gene FISH map for analyzing homoeologous relationship and chromosomal rearrangements within the Triticeae. Theoretical and Applied Genetics, 127(3), 715-730. https://doi.org/10.1007/s00122-013-2253-z
dc.contributor.authoreid tatianad en_US
dc.contributor.authoreid friebe en_US
dc.contributor.authoreid bsgill en_US
dc.description.version Article: Version of Record


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This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. Except where otherwise noted, the use of this item is bound by the following: This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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