Cytological and comparative proteomic analyses on male sterility in Brassica napus L. induced by the chemical hybridization agent monosulphuron ester sodium

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dc.contributor.author Cheng, Yufeng
dc.contributor.author Wang, Qian
dc.contributor.author Li, Zhanjie
dc.contributor.author Cui, Jianmin
dc.contributor.author Hu, Shengwu
dc.contributor.author Zhao, Huixian
dc.contributor.author Chen, Ming-Shun
dc.date.accessioned 2014-02-18T22:29:41Z
dc.date.available 2014-02-18T22:29:41Z
dc.date.issued 2014-02-18
dc.identifier.uri http://hdl.handle.net/2097/17178
dc.description Citation: Cheng Y, Wang Q, Li Z, Cui J, Hu S, et al. (2013) Cytological and Comparative Proteomic Analyses on Male Sterility in Brassica napus L. Induced by the Chemical Hybridization Agent Monosulphuron Ester Sodium. PLoS ONE 8(11): e80191. doi:10.1371/journal.pone.0080191
dc.description.abstract Male sterility induced by a chemical hybridization agent (CHA) is an important tool for utilizing crop heterosis. Monosulphuron ester sodium (MES), a new acetolactate synthase-inhibitor herbicide belonging to the sulphonylurea family, has been developed as an effective CHA to induce male sterility in rapeseed (Brassica napus L.). To understand MES-induced male sterility in rapeseed better, comparative cytological and proteomic analyses were conducted in this study. Cytological analysis indicated that defective tapetal cells and abnormal microspores were gradually generated in the developing anthers of MES-treated plants at various development stages, resulting in unviable microspores and male sterility. A total of 141 differentially expressed proteins between the MES-treated and control plants were revealed, and 131 of them were further identified by MALDI-TOF/TOF MS. Most of these proteins decreased in abundance in tissues of MES-treated rapeseed plants, and only a few increased. Notably, some proteins were absent or induced in developing anthers after MES treatment. These proteins were involved in several processes that may be crucial for tapetum and microspore development. Down-regulation of these proteins may disrupt the coordination of developmental and metabolic processes, resulting in defective tapetum and abnormal microspores that lead to male sterility in MES-treated plants. Accordingly, a simple model of CHA-MES-induced male sterility in rapeseed was established. This study is the first cytological and dynamic proteomic investigation on CHA-MES-induced male sterility in rapeseed, and the results provide new insights into the molecular events of male sterility. en_US
dc.language.iso en_US en_US
dc.relation.uri https://doi.org/10.1371/journal.pone.0080191 en_US
dc.rights This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
dc.rights.uri https://creativecommons.org/share-your-work/public-domain/cc0/
dc.subject Brassica napus L. en_US
dc.subject Monosulphuron ester sodium en_US
dc.subject Rapeseed en_US
dc.subject Male sterility en_US
dc.title Cytological and comparative proteomic analyses on male sterility in Brassica napus L. induced by the chemical hybridization agent monosulphuron ester sodium en_US
dc.type Text en_US
dc.date.published 2013 en_US
dc.citation.doi 10.1371/journal.pone.0080191 en_US
dc.citation.issn 1932-6203
dc.citation.issue 11 en_US
dc.citation.jtitle PLoS ONE en_US
dc.citation.spage e80191 en_US
dc.citation.volume 8 en_US
dc.citation Cheng Y, Wang Q, Li Z, Cui J, Hu S, et al. (2013) Cytological and Comparative Proteomic Analyses on Male Sterility in Brassica napus L. Induced by the Chemical Hybridization Agent Monosulphuron Ester Sodium. PLoS ONE 8(11): e80191. doi:10.1371/journal.pone.0080191
dc.contributor.authoreid mchen en_US
dc.description.version Article: Publisher version


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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. Except where otherwise noted, the use of this item is bound by the following: This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

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