Analysis of EST’s encoding pea aphid Acyrthosiphon pisum C002 & the effect of armet transcript knockdown in Tribolium castaneum

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dc.contributor.author Heerman, Matthew C.
dc.date.accessioned 2014-02-03T16:08:23Z
dc.date.available 2014-02-03T16:08:23Z
dc.date.issued 2014-02-03
dc.identifier.uri http://hdl.handle.net/2097/17148
dc.description.abstract Aphids mount a remarkable salivary secretion to overcome plant host defenses. Our group has previously reported a gene unique to aphids enriched in the salivary glands of the pea aphid A. pisum, C002, which is required for successful feeding on its host plant Vicia fava. Here I present an analysis of genetic variation within the available EST data for C002 in pea aphids. From 596 total ESTs, 332 are full-length, and segregate into 8 validated haplotypes based on the criteria I set in place to access the quality of EST data. Additionally, Armet, is a putative multi-functional gene implicated as a neurotrophic factor during development, and as a part of the unfolded protein response during stress. I employ RNA interference in the model organism T. castaneum to determine the effect of transcript knockdown during development from early in-star larval stages, through pupation, and its effect on adult emergence. I report that knockdown of Armet transcript significantly hinders the ability for beetles to emerge from the pupae. en_US
dc.language.iso en_US en_US
dc.publisher Kansas State University en
dc.subject RNAi en_US
dc.subject Armet en_US
dc.subject Tribolium castaneum en_US
dc.subject Neurotrophic factor en_US
dc.title Analysis of EST’s encoding pea aphid Acyrthosiphon pisum C002 & the effect of armet transcript knockdown in Tribolium castaneum en_US
dc.type Thesis en_US
dc.description.degree Master of Science en_US
dc.description.level Masters en_US
dc.description.department Department of Biochemistry en_US
dc.description.advisor Gerald Reeck en_US
dc.subject.umi Biochemistry (0487) en_US
dc.subject.umi Genetics (0369) en_US
dc.date.published 2012 en_US
dc.date.graduationmonth December en_US


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