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Browsing Biology by Title

Now showing items 168-187 of 208

RNAi Trigger Delivery into Anopheles gambiae Pupae

Regna, K.; Harrison, R. M.; Heyse, S. A.; Chiles, T. C.; Michel, Kristin; Muskavitch, M. A. T.

RNA interference (RNAi), a naturally occurring phenomenon in eukaryotic organisms, is an extremely valuable tool that can be utilized in the laboratory for functional genomic studies. The ability to knockdown individual ...
The role of N-glycosylation in folding, trafficking, and functionality of lysosomal protein CLN5

Moharir, Akshay; Peck, Sun H.; Budden, Theodore; Lee, Stella Y. (2013-10-11)

CLN5 is a soluble lysosomal protein with unknown function. Mutations in CLN5 lead to neuronal ceroid lipofuscinosis, a group of inherited neurodegenerative disorders that mainly affect children. CLN5 has eight potential ...
RPFdb: a database for genome wide information of translated mRNA generated from ribosome profiling

Xie, S. Q.; Nie, P.; Wang, Y.; Wang, H. W.; Li, H. Y.; Yang, Zhilong L.; Liu, Y. Z.; Ren, J.; Xie, Z.

Translational control is crucial in the regulation of gene expression and deregulation of translation is associated with a wide range of cancers and human diseases. Ribosome profiling is a technique that provides genome ...
Saturation of NO3- uptake in prairie streams as a function of acute and chronic N exposure.

O'Brien, Jonathan M.; Dodds, Walter K. (2011-11-04)

We conducted a series of stepwise NO3- additions to investigate the response of NO3- uptake to short-term (acute) changes in N concentration in 3 prairie streams. Observed NO3- uptake rates increased with short-term ...
Savanna Tree Seedlings are Physiologically Tolerant to Nighttime Freeze Events

O'Keefe, K.; Nippert, Jesse B.; Swemmer, A. M.

Freeze events can be important disturbances in savanna ecosystems, yet the interactive effect of freezing with other environmental drivers on plant functioning is unknown. Here, we investigated physiological responses of ...
Scraping the bottom of the barrel: are rare high throughput sequences artifacts?

Brown, Shawn Paul; Veach, Allison M.; Rigdon-Huss, Anne R.; Grond, Kirsten; Lickteig, Spencer K.; Lothamer, Kale M.; Oliver, Alena K.; Jumpponen, Ari M.

Metabarcoding data generated using next-generation sequencing (NGS) technologies are overwhelmed with rare taxa and skewed in Operational Taxonomic Unit (OTU) frequencies comprised of few dominant taxa. Low frequency OTUs ...
Seed source has variable effects on species, communities, and ecosystem properties in grassland restorations

Carter, Daniel L.; Blair, John M. (2013-09-11)

Research to date regarding the relative advantages of local versus non-local sources of plant material for restoration has produced equivocal results. This research has typically focused on the performance of individual ...
Septate endophyte colonization and host responses of grasses and forbs native to a tallgrass prairie

Mandyam, Keerthi; Fox, Chad; Jumpponen, Ari M. (2012-05-11)

Native tallgrass prairies support distinct dark septate endophyte (DSE) communities exemplified by periconia macrospinosa and Microdochium sp. that were recently identified as common root symbionts in this system. Since ...
Sequence of the Gonium pectorale mating locus reveals a complex and dynamic history of changes in volvocine algal mating haplotypes

Hamaji, T.; Mogi, Y.; Ferris, P. J.; Mori, T.; Miyagishima, S.; Kabeya, Y.; Nishimura, Y.; Toyoda, A.; Noguchi, H.; Fujiyama, A.; Olson, Bradley J.; Marriage, Tara N.; Nishii, I.; Umen, J. G.

Sex-determining regions (SDRs) or mating-type (MT) loci in two sequenced volvocine algal species, Chlamydomonas reinhardtii and Volvox carteri, exhibit major differences in size, structure, gene content, and gametolog ...
Sequential eIF5 binding to the charged disordered segments of eIF4G and eIF2β stabilizes the 48S pre-initiation complex and promotes its shift to the initiation mode

Singh, Chingakham Ranjit; Watanabe, Ryosuke; Chowdhury, Wasimul Q.; Hiraishi, Hiroyuki; Murai, Marcelo J.; Yamamoto, Yasufumi; Miles, David; Ikeda, Yuka; Asano, Masayo; Asano, Katsura (2012-12-04)

During translation initiation in Saccharomyces cerevisiae, an Arg- and Ser-rich segment (RS1 domain) of eIF4G and the Lys-rich segment (K-boxes) of eIF2β bind three common partners, eIF5, eIF1 and mRNA. Here we report that ...
Sex-specific post-translational regulation of the gamete fusogen GCS1 in the isogamous volvocine alga Gonium pectorale

Kawai-Toyooka, Hiroko; Mori, Toshiyuki; Hamaji, Takashi; Suzuki, Masahiro; Olson, Bradley J.; Uemura, Tomohiro; Ueda, Takashi; Nakano, Akihiko; Toyoda, Atsushi; Fujiyama, Asao; Nozaki, Hisayoshi (2014-07-22)

Male and female, generally defined based on differences in gamete size and motility, likely have multiple independent origins, appearing to have evolved from isogamous organisms in various eukaryotic lineages. Recent studies ...
Shared functions of plant and mammalian StAR-related lipid transfer (START) domains in modulating transcription factor activity

Schrick, Kathrin; Bruno, Michael; Khosla, Aashima; Cox, Paige N.; Marlatt, Sara A.; Roque, Remigio A.; Nguyen, Henry C.; He, Cuiwen; Snyder, Michael P.; Singh, Daljit; Yadav, Gitanjali (2015-03-18)

Background: Steroidogenic acute regulatory protein (StAR)-related lipid transfer (START) domains were first identified from mammalian proteins that bind lipid/sterol ligands via a hydrophobic pocket. In plants, predicted ...
Space use by female Greater Prairie-Chickens in response to wind energy development

Winder, V. L.; McNew, L. B.; Gregory, A. J.; Hunt, L. M.; Wisely, Samantha M.; Sandercock, Brett K. (2014-04-09)

Wind energy development is targeted to meet 20% of U.S. energy demand by 2030. In Kansas, optimal sites for wind energy development often overlap with preferred habitats of Greater Prairie-Chickens (Tympanuchus cupido), a ...
Spatiotemporal scaling of North American continental interior wetlands: implications for shorebird conservation

Albanese, Gene; Davis, Craig A.; Compton, Bradley W. (2013-05-06)

Within interior North America, erratic weather patterns and heterogeneous wetland complexes cause wide spatio-temporal variation in the resources available to migrating shorebirds. Identifying the pattern-generating ...
Species and population level molecular profiling reveals cryptic recombination and emergent asymmetry in the dimorphic mating locus of C. reinhardtii

De Hoff, Peter L.; Ferris, Patrick; Olson, Bradley J.; Miyagi, Ayano; Geng, Sa; Umen, James G. (2013-10-07)

Heteromorphic sex-determining regions or mating-type loci can contain large regions of non-recombining sequence where selection operates under different constraints than in freely recombining autosomal regions. Detailed ...
Stability of grassland soil C and N pools despite 25years of an extreme climatic and disturbance regime

Wilcox, K. R.; Blair, John M.; Knapp, A. K.

Global changes are altering many important drivers of ecosystem functioning, with precipitation amount and disturbance frequency being especially important. Carbon (C) and nitrogen (N) pools are key contemporary attributes ...
Starvation Induces Proteasome Autophagy with Different Pathways for Core and Regulatory Particles

Waite, Kenrick A.; De La Mota-Peynado, Alina; Vontz, Gabrielle; Roelofs, Jeroen

The proteasome is responsible for the degradation of many cellular proteins. If and how this abundant and normally stable complex is degraded by cells is largely unknown. Here we show that in yeast, upon nitrogen starvation, ...
Stream microbial diversity in response to environmental changes: review and synthesis of existing research

Zeglin, Lydia H.
Structure of trophic and mutualistic networks across broad environmental gradients

Welti, E. A. R.; Joern, Anthony
Surprises and insights from long-term aquatic data sets and experiments

Dodds, Walter K.; Robinson, Christopher T.; Gaiser, Evelyn E.; Hansen, Gretchen J. A.; Powell, Heather; Smith, Joseph M.; Morse, Nathaniel B.; Johnson, Sherri L.; Gregory, Stanley V.; Bell, Tisza; Kratz, Timothy K.; McDowell, William H. (2012-11-14)

Long-term research on freshwater ecosystems provides insights that can be difficult to obtain from other approaches. Widespread monitoring of ecologically relevant water-quality parameters spanning decades can facilitate ...