Sequence organization and evolutionary dynamics of Brachypodium-specific centromere retrotransposons

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dc.contributor.author Qi, L. L.
dc.contributor.author Wu, J. J.
dc.contributor.author Friebe, Bernd R.
dc.contributor.author Qian, C.
dc.contributor.author Gu, Y. Q.
dc.contributor.author Fu, D. L.
dc.contributor.author Gill, Bikram S.
dc.date.accessioned 2013-11-07T22:20:09Z
dc.date.available 2013-11-07T22:20:09Z
dc.date.issued 2013-08-17
dc.identifier.uri http://hdl.handle.net/2097/16757
dc.description Citation: Qi, L., . . . Gill, B. (2013). Sequence organization and evolutionary dynamics of Brachypodium-specific centromere retrotransposons. Chromosome Research, 21, 507-521. https://doi.org/10.1007/s10577-013-9378-4
dc.description.abstract Brachypodium distachyon is a wild annual grass belonging to the Pooideae, more closely related to wheat, barley, and forage grasses than rice and maize. As an experimental model, the completed genome sequence of B. distachyon provides a unique opportunity to study centromere evolution during the speciation of grasses. Centromeric satellite sequences have been identified in B. distachyon, but little is known about centromeric retrotransposons in this species. In the present study, bacterial artificial chromosome (BAC)-fluorescence in situ hybridization was conducted in maize, rice, barley, wheat, and rye using B. distachyon (Bd) centromere-specific BAC clones. Eight Bd centromeric BAC clones gave no detectable fluorescence in situ hybridization (FISH) signals on the chromosomes of rice and maize, and three of them also did not yield any FISH signals in barley, wheat, and rye. In addition, four of five Triticeae centromeric BAC clones did not hybridize to the B. distachyon centromeres, implying certain unique features of Brachypodium centromeres. Analysis of Brachypodium centromeric BAC sequences identified a long terminal repeat (LTR)-centromere retrotransposon of B. distachyon (CRBd1). This element was found in high copy number accounting for 1.6 % of the B. distachyon genome, and is enriched in Brachypodium centromeric regions. CRBd1 accumulated in active centromeres, but was lost from inactive ones. The LTR of CRBd1 appears to be specific to B. distachyon centromeres. These results reveal different evolutionary events of this retrotransposon family across grass species. en_US
dc.language.iso en_US en_US
dc.relation.uri https://doi.org/10.1007/s10577-013-9378-4 en_US
dc.rights This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). en_US
dc.rights.uri https://www.springer.com/gp/open-access/publication-policies/self-archiving-policy
dc.rights.uri https://rightsstatements.org/page/InC/1.0/?language=en
dc.subject Brachypodium distachyon en_US
dc.subject Centromere retrotransposons en_US
dc.subject Evolution en_US
dc.title Sequence organization and evolutionary dynamics of Brachypodium-specific centromere retrotransposons en_US
dc.type Text en_US
dc.date.published 2013 en_US
dc.citation.doi 10.1007/s10577-013-9378-4 en_US
dc.citation.epage 521 en_US
dc.citation.issn 0967-3849
dc.citation.issue 5 en_US
dc.citation.jtitle Chromosome Research en_US
dc.citation.spage 507 en_US
dc.citation.volume 21 en_US
dc.citation Qi, L., . . . Gill, B. (2013). Sequence organization and evolutionary dynamics of Brachypodium-specific centromere retrotransposons. Chromosome Research, 21, 507-521. https://doi.org/10.1007/s10577-013-9378-4
dc.contributor.authoreid friebe en_US
dc.contributor.authoreid bsgill en_US
dc.description.version Article: Accepted Manuscript


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