Intestinal lipid alterations occur prior to antibody-induced prostaglandin E2 production in a mouse model of ischemia/reperfusion

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dc.contributor.author Sparkes, Byron L.
dc.contributor.author Archer Slone, Emily E.
dc.contributor.author Roth, Mary R.
dc.contributor.author Welti, Ruth
dc.contributor.author Fleming, Sherry D.
dc.date.accessioned 2013-10-16T16:35:48Z
dc.date.available 2013-10-16T16:35:48Z
dc.date.issued 2013-10-16
dc.identifier.uri http://hdl.handle.net/2097/16677
dc.description.abstract Ischemia/reperfusion (IR) induced injury results in significant tissue damage in wild-type, but not antibody-deficient, Rag-1-/- mice. However, Rag-1-/- mice sustain intestinal damage after administration of wild-type antibodies or naturally occurring, specific anti-phospholipid related monoclonal antibodies, suggesting involvement of a lipid antigen. We hypothesized that IR initiates metabolism of cellular lipids, resulting in production of an antigen recognized by anti-phospholipid antibodies. At multiple time points after Sham or IR treatment, lipids extracted from mouse jejunal sections were analyzed by electrospray ionization triple quadrupole mass spectrometry. Within 15 min of reperfusion, IR induced significantly more lysophosphatidylcholine (lysoPC), lysophosphatidylglycerol (lysoPG) and free arachidonic acid (AA) production than Sham treatment. While lysoPC, lysoPG, and free AA levels were similar in C57Bl/6 (wild-type) and Rag-1-/- mice, IR led to Cox-2 activation and prostaglandin E[subscript 2] (PGE[subscript 2]) production in wild-type, but not in the antibody-deficient, Rag-1-/- mice. Administration of wild-type antibodies to Rag-1-/- mice restored PGE[subscript 2] production and intestinal damage. These data indicate that IR-induced intestinal damage requires antibodies for Cox-2 stimulated PGE[subscript 2] production but not for production of lysoPC and free AA. en_US
dc.language.iso en_US en_US
dc.relation.uri http://www.sciencedirect.com/science/article/pii/S1388198110000053 en_US
dc.subject Mass spectrometry en_US
dc.subject Mice en_US
dc.subject Mouse en_US
dc.subject Intestine en_US
dc.subject Lipidomics en_US
dc.title Intestinal lipid alterations occur prior to antibody-induced prostaglandin E2 production in a mouse model of ischemia/reperfusion en_US
dc.type Article (author version) en_US
dc.date.published 2010 en_US
dc.citation.doi doi:10.1016/j.bbalip.2010.01.004 en_US
dc.citation.epage 525 en_US
dc.citation.issue 4 en_US
dc.citation.jtitle Biochimica et Biophysica Acta en_US
dc.citation.spage 517 en_US
dc.citation.volume 1801 en_US
dc.contributor.authoreid mrroth en_US
dc.contributor.authoreid welti en_US
dc.contributor.authoreid sdflemin en_US


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