Both flagella and F4 fimbriae from F4ac+ enterotoxigenic Escherichia coli contribute to attachment to IPEC-J2 cells in vitro

K-REx Repository

Show simple item record

dc.contributor.author Zhou, Mingxu
dc.contributor.author Duan, Qiangde
dc.contributor.author Zhu, Xiaofang
dc.contributor.author Guo, Zhiyan
dc.contributor.author Li, Yinchau
dc.contributor.author Hardwidge, Philip R.
dc.contributor.author Zhu, Guoqiang
dc.date.accessioned 2013-09-11T19:53:14Z
dc.date.available 2013-09-11T19:53:14Z
dc.date.issued 2013-09-11
dc.identifier.uri http://hdl.handle.net/2097/16421
dc.description.abstract The role of flagella in the pathogenesis of F4ac[superscript +] Enterotoxigenic Escherichia coli (ETEC) mediated neonatal and post-weaning diarrhea (PWD) is not currently understood. We targeted the reference C83902 ETEC strain (O8:H19:F4ac[superscript +] LT[superscript +] STa[superscript +] STb[superscript +]), to construct isogenic mutants in the fliC (encoding the major flagellin protein), motA (encoding the flagella motor), and faeG (encoding the major subunit of F4 fimbriae) genes. Both the ΔfliC and ΔfaeG mutants had a reduced ability to adhere to porcine intestinal epithelial IPEC-J2 cells. F4 fimbriae expression was significantly down-regulated after deleting fliC, which revealed that co-regulation exists between flagella and F4 fimbriae. However, there was no difference in adhesion between the ΔmotA mutant and its parent strain. These data demonstrate that both flagella and F4 fimbriae are required for efficient F4ac[superscript +] ETEC adhesion in vitro. en_US
dc.language.iso en_US en_US
dc.relation.uri http://www.veterinaryresearch.org/content/44/1/30 en_US
dc.subject Escherichia coli en_US
dc.subject Neonatal pigs en_US
dc.subject Young pigs en_US
dc.subject Diarrhea en_US
dc.title Both flagella and F4 fimbriae from F4ac+ enterotoxigenic Escherichia coli contribute to attachment to IPEC-J2 cells in vitro en_US
dc.type Article (publisher version) en_US
dc.date.published 2013 en_US
dc.citation.doi doi:10.1186/1297-9716-44-30 en_US
dc.citation.jtitle Veterinary Research en_US
dc.citation.spage 30 en_US
dc.citation.volume 44 en_US
dc.contributor.authoreid philiphardwidge en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search K-REx


Advanced Search

Browse

My Account

Statistics








Center for the

Advancement of Digital

Scholarship

cads@k-state.edu