Serine and cysteine protease-like genes in the genome of a gall midge and their interactions with host plant genotypes

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dc.contributor.author Chen, Hang
dc.contributor.author Zhu, Yu Cheng
dc.contributor.author Whitworth, R. Jeffery
dc.contributor.author Reese, John C.
dc.contributor.author Chen, Ming-Shun
dc.date.accessioned 2013-09-05T20:38:13Z
dc.date.available 2013-09-05T20:38:13Z
dc.date.issued 2013-09-05
dc.identifier.uri http://hdl.handle.net/2097/16407
dc.description.abstract Proteases play important roles in a wide range of physiological processes in organisms. For plant-feeding insects, digestive proteases are targets for engineering protease inhibitors for pest control. In this study, we identified 105 putative serine- and cysteine-protease genes from the genome of the gall midge Mayetiola destructor (commonly known as Hessian fly), a destructive pest of wheat. Among the genes, 31 encode putative trypsins, 18 encode putative chymotrypsins, seven encode putative cysteine proteases, and the remaining may encode either other proteases or protease homologues. Developmental stage- and tissue-specific expression profiles of the genes encoding putative trypsins, chymotrypsins, and cysteine proteases were determined by quantitative reverse-transcription PCR. Comparative analyses of stage- and tissue-specific expression patterns suggested that several genes are likely to encode digestive proteases in the M. destructor larval gut, including genes encoding putative trypsins MDP3, MDP5, MDP9, MDP24, MDP48, MDP51, MDP57, MDP61, MDP71, and MDP90; genes encoding putative chymotrypsins MDP1, MDP7, MDP8, MDP18, MDP19, and MDP20; and genes encoding putative cysteine proteases MDP95 and MDP104. The expression of some protease genes was affected by plant genotypes. Genes encoding trypsins MDP3, MDP9, and MPD23, chymotrypsins MDP20 and MDP21, and cysteine proteases MDP99 and MDP104 were upregulated in M. destructor larvae feeding in resistant plants, whereas genes encoding trypsins MDP12, MDP24, and MDP33, and chymotrypsins MDP8, MDP15, and MDP16 were downregulated in M. destructor larvae feeding in resistant plants. This study provides a foundation for further comparative studies on proteases in different insects, and further characterization of M. destructor digestive proteases and their interactions with host plants, as well as potential targets for transgenic wheat plants. en_US
dc.language.iso en_US en_US
dc.relation.uri http://www.sciencedirect.com/science/article/pii/S0965174813001008 en_US
dc.subject Hessian fly genome en_US
dc.subject Mayetiola destructor en_US
dc.subject Serine protease en_US
dc.subject Cysteine protease en_US
dc.subject Gut proteinases en_US
dc.title Serine and cysteine protease-like genes in the genome of a gall midge and their interactions with host plant genotypes en_US
dc.type Article (author version) en_US
dc.date.published 2013 en_US
dc.citation.doi doi:10.1016/j.ibmb.2013.05.006 en_US
dc.citation.epage 711 en_US
dc.citation.issue 8 en_US
dc.citation.jtitle Insect Biochemistry and Molecular Biology en_US
dc.citation.spage 701 en_US
dc.citation.volume 43 en_US
dc.contributor.authoreid hangc en_US
dc.contributor.authoreid jwhitwor en_US
dc.contributor.authoreid jreese en_US
dc.contributor.authoreid mchen en_US


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