Patatin-related phospholipase pPLAIIIδ increases seed oil content with long-chain fatty acids in Arabidopsis

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Show simple item record Li, Maoyin Bahn, Sung Chul Fan, Chuchuan Li, Jia Phan, Tien Ortiz, Michael Roth, Mary R. Welti, Ruth Jaworski, Jan Wang, Xuemin 2013-07-26T20:30:01Z 2013-07-26T20:30:01Z 2013-07-26
dc.description.abstract The release of fatty acids from membrane lipids has been implicated in various metabolic and physiological processes, but in many cases, the enzymes involved and their functions in plants remain unclear. Patatin-related phospholipase As (pPLAs) constitute a major family of acyl-hydrolyzing enzymes in plants. Here, we show that pPLAIIIδ promotes the production of triacylglycerols with 20- and 22-carbon fatty acids in Arabidopsis (Arabidopsis thaliana). Of the four pPLAIIIs (α, β, γ, δ), only pPLAIIIδ gene knockout results in a decrease in seed oil content, and pPLAIIIδ is most highly expressed in developing embryos. The overexpression of pPLAIIIδ increases the content of triacylglycerol and 20- and 22-carbon fatty acids in seeds with a corresponding decrease in 18-carbon fatty acids. Several genes in the glycerolipid biosynthetic pathways are up-regulated in pPLAIIIδ-overexpressing siliques. pPLAIIIδ hydrolyzes phosphatidylcholine and also acyl-coenzyme A to release fatty acids. pPLAIIIδ-overexpressing plants have a lower level, whereas pPLAIIIδ knockout plants have a higher level, of acyl-coenzyme A than the wild type. Whereas seed yield decreases in transgenic plants that ubiquitously overexpress pPLAIIIδ, seed-specific overexpression of pPLAIIIδ increases seed oil content without any detrimental effect on overall seed yield. These results indicate that pPLAIIIδ-mediated phospholipid turnover plays a role in fatty acid remodeling and glycerolipid production. en_US
dc.language.iso en_US en_US
dc.relation.uri en_US
dc.subject Fatty acids en_US
dc.subject Patatin-related phospholipase As en_US
dc.subject Acyl-hydrolyzing enzymes en_US
dc.subject pPLAIIIδ en_US
dc.subject Arabidopsis thaliana en_US
dc.title Patatin-related phospholipase pPLAIIIδ increases seed oil content with long-chain fatty acids in Arabidopsis en_US
dc.type Article (publisher version) en_US 2013 en_US
dc.citation.doi doi:10.1104/pp.113.216994 en_US
dc.citation.epage 51 en_US
dc.citation.issue 1 en_US
dc.citation.jtitle Plant Physiology en_US
dc.citation.spage 39 en_US
dc.citation.volume 162 en_US
dc.contributor.authoreid mrroth en_US
dc.contributor.authoreid welti en_US

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