Soil-based systemic delivery and phyllosphere in vivo propagation of bacteriophages: Two possible strategies for improving bacteriophage persistence for plant disease control

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dc.contributor.author Iriarte, Fanny B.
dc.contributor.author Obradović, Aleksa
dc.contributor.author Wernsing, Mine H.
dc.contributor.author Jackson, Lee E.
dc.contributor.author Balogh, Botond
dc.contributor.author Hong, Jason A.
dc.contributor.author Momol, M. Timur
dc.contributor.author Jones, Jeffrey B.
dc.contributor.author Vallad, Gary E.
dc.date.accessioned 2013-07-15T13:33:54Z
dc.date.available 2013-07-15T13:33:54Z
dc.date.issued 2012-10-01
dc.identifier.uri http://hdl.handle.net/2097/15974
dc.description Citation: Iriarte, F., Obradovic, A., Wernsing, M., . . . Vallad, G. (2012). Soil-based systemic delivery and phyllosphere in vivo propagation of bacteriophages. Bacterioophage, 2(4), 215-224. https://doi.org/10.4161/bact.23530
dc.description.abstract Soil-based root applications and attenuated bacterial strains were evaluated as means to enhance bacteriophage persistence on plants for bacterial disease control. In addition, the systemic nature of phage applied to tomato roots was also evaluated. Several experiments were conducted applying either single phages or phage mixtures specific for Ralstonia solanacearum, Xanthomonas perforans or X. euvesicatoria to soil surrounding tomato plants and measuring the persistence and translocation of the phages over time. In general, all phages persisted in the roots of treated plants and were detected in stems and leaves; although phage level varied and persistence in stems and leaves was at a much lower level compared with persistence in roots. Bacterial wilt control was typically best if the phage or phage mixtures were applied to the soil surrounding tomatoes at the time of inoculation, less effective if applied 3 days before inoculation, and ineffective if applied 3 days after inoculation. The use of an attenuated X. perforans strain was also evaluated to improve the persistence of phage populations on tomato leaf surfaces. In greenhouse and field experiments, foliar applications of an attenuated mutant X. perforans 91-118:ΔOPGH strain prior to phage applications significantly improved phage persistence on tomato foliage compared with untreated tomato foliage. Both the soil-based bacteriophage delivery and the use of attenuated bacterial strains improved bacteriophage persistence on respective root and foliar tissues, with evidence of translocation with soil-based bacteriophage applications. Both strategies could lead to improved control of bacterial pathogens on plants. en_US
dc.language.iso en_US en_US
dc.relation.uri https://doi.org/10.4161/bact.23530 en_US
dc.rights This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.rights.uri https://rightsstatements.org/page/InC/1.0/?language=en
dc.rights.uri https://authorservices.taylorandfrancis.com/publishing-open-access/#Repositories
dc.subject Bacteriophage en_US
dc.subject Biocontrol en_US
dc.subject Phage en_US
dc.subject Tomato en_US
dc.title Soil-based systemic delivery and phyllosphere in vivo propagation of bacteriophages: Two possible strategies for improving bacteriophage persistence for plant disease control en_US
dc.type Text en_US
dc.date.published 2012 en_US
dc.citation.doi 10.4161/bact.23530 en_US
dc.citation.epage 224 en_US
dc.citation.issn 2159-7073
dc.citation.issue 4 en_US
dc.citation.jtitle Bacteriophage en_US
dc.citation.spage 215 en_US
dc.citation.volume 2 en_US
dc.citation Iriarte, F., Obradovic, A., Wernsing, M., . . . Vallad, G. (2012). Soil-based systemic delivery and phyllosphere in vivo propagation of bacteriophages. Bacterioophage, 2(4), 215-224. https://doi.org/10.4161/bact.23530
dc.contributor.authoreid firiarte en_US
dc.description.version Article: Version of Record


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This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). Except where otherwise noted, the use of this item is bound by the following: This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).

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