Bradykinin-stimulated cyclooxygenase activity stimulates porcine and human vas deferens epithelial anion secretion in vitro

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dc.contributor.author Pierucci-Alves, Fernando
dc.contributor.author Schultz, Bruce D.
dc.date.accessioned 2013-06-27T19:41:48Z
dc.date.available 2013-06-27T19:41:48Z
dc.date.issued 2013-06-27
dc.date.issued 2008-09-01
dc.identifier.uri http://hdl.handle.net/2097/15940
dc.description.abstract Epithelia lining the male reproductive duct modulate fertility by altering the luminal environment to which sperm are exposed. Although vas deferens epithelial cells reportedly express high levels of cyclooxygenases (Ptgs), and activation of bradykinin (BK) receptors can lead to upregulation of PTGS activity in epididymal epithelia, it remains unknown whether BKs and/or PTGSs have any role in modulating epithelial ion transport across vas deferens epithelia. Porcine and human vas deferens epithelial cell primary cultures and the PVD9902 cell line responded to lysylbradykinin with an increase in short circuit current (I[subscript sc]; indicating net anion secretion), an effect that was 60-93% reduced by indomethacin. The BK effect was inhibited by the B2 receptor subtype (BDKRB2) antagonist HOE140, while the B1 receptor subtype agonist des-Arg[superscript 9]-BK had no effect. BDKRB2 immunoreactivity was documented in most epithelial cells composing the native epithelium and on Western blots derived from cultured cells. Gene expression analysis revealed that the PTGS2 transcript is 20 times more abundant than its PTGS1 counterpart in cultured porcine vas deferens epithelia and that BDKRB2 mRNA is likewise highly expressed. Subsequent experiments revealed that prostaglandin E2, 1-OH prostaglandin E1 (prostaglandin E receptor 4 [PTGER4] agonist) and butaprost (PTGER2 agonist) increase I[subscript sc] in a concentration-dependent manner, whereas sulprostone (mixed PTGER1 and PTGER3 agonist) produced no change in I[subscript sc]. These results demonstrate that autacoids can affect epithelial cells to acutely modulate the luminal environment to which sperm are exposed in the vas deferens by enhancing PTGS activity leading to the production of prostaglandins that act at PTGER4 and/or PTGER2 to induce or enhance anion secretion. en_US
dc.language.iso en_US en_US
dc.relation.uri http://www.biolreprod.org/content/79/3/501.full en_US
dc.rights Permission to archive granted by the Society for the Study of Reproduction, September 20, 2012. en_US
dc.subject Male reproductive tract en_US
dc.subject Mechanisms of hormone action en_US
dc.subject Polypeptide receptors en_US
dc.subject Signal transduction en_US
dc.subject Vas deferens en_US
dc.title Bradykinin-stimulated cyclooxygenase activity stimulates porcine and human vas deferens epithelial anion secretion in vitro en_US
dc.title.alternative Bradykinin-stimulated cyclooxygenase activity stimulates vas deferens epithelial anion secretion in vitro in swine and humans en_US
dc.type Article (author version) en_US
dc.date.published 2008 en_US
dc.citation.doi 10.1095/biolreprod.107.066910 en_US
dc.citation.epage 509 en_US
dc.citation.issue 3 en_US
dc.citation.jtitle Biology of Reproduction en_US
dc.citation.spage 501 en_US
dc.citation.volume 79 en_US
dc.contributor.authoreid fpalves en_US
dc.contributor.authoreid bschultz en_US


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