Differential proteomic analysis reveals increased cathelicidin expression in porcine bronchoalveolar lavage fluid after an Actinobacillus pleuropneumoniae infection

K-REx Repository

Show simple item record

dc.contributor.author Hennig-Pauka, Isabel
dc.contributor.author Jacobsen, Ilse
dc.contributor.author Blecha, Frank
dc.contributor.author Waldmann, Karl-Heinz
dc.contributor.author Gerlach, Gerald-Friedrich
dc.date.accessioned 2013-05-29T20:37:25Z
dc.date.available 2013-05-29T20:37:25Z
dc.date.issued 2013-05-29
dc.date.issued 2015-12-13
dc.identifier.uri http://hdl.handle.net/2097/15863
dc.description.abstract Accurate definition of respiratory health in pigs is an important problem for swine producers and veterinarians. In an approach to identify potential biomarkers, two-dimensional gel electrophoresis and mass spectrometry on bronchoalveolar lavage fluid (BALF)-derived proteins from pigs experimentally infected with Actinobacillus pleuropneumoniae were performed at different time points post infection. Mock-infected pigs were used as a control. It was shown that the antimicrobial peptides, prophenin-2 and PR-39, and the calcium-binding protein calgranulin C were reproducibly upregulated in BALF of pigs chronically infected with A. pleuropneumoniae. Concentrations of PR-39 were significantly (p < 0.05) increased in BALF (median of 4.8 nM) but not in serum (median of 2.5 nM) on day 21 after infection. A Receiver Operating Characteristics (ROC) plot showed that PR-39 in BALF is an accurate and easily accessible marker to detect clinically healthy pigs convalescent from an experimental A. pleuropneumoniae infection. These results imply that PR-39 might have a potential as a general biomarker to determine porcine respiratory health. en_US
dc.language.iso en_US en_US
dc.relation.uri http://doi.org/10.1051/vetres:2005043 en_US
dc.rights The original publication is available at http://www.vetres.org/ en_US
dc.subject Antimicrobial peptide en_US
dc.subject PR-39 en_US
dc.subject BALF en_US
dc.subject Porcine respiratory tract en_US
dc.subject Bronchoalveolar lavage fluid en_US
dc.title Differential proteomic analysis reveals increased cathelicidin expression in porcine bronchoalveolar lavage fluid after an Actinobacillus pleuropneumoniae infection en_US
dc.type Article (publisher version) en_US
dc.date.published 2006 en_US
dc.citation.doi 10.1051/vetres:2005043 en_US
dc.citation.epage 87 en_US
dc.citation.issue 1 en_US
dc.citation.jtitle Veterinary Research en_US
dc.citation.spage 75 en_US
dc.citation.volume 37 en_US
dc.contributor.authoreid blecha en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search K-REx


Advanced Search

Browse

My Account

Statistics








Center for the

Advancement of Digital

Scholarship

cads@k-state.edu