Toll-like receptor (TLR) 2 and TLR4 gene expression in canine heart

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dc.contributor.author Linde, Annika
dc.contributor.author Blecha, Frank
dc.contributor.author Melgarejo, Tonatiuh
dc.date.accessioned 2013-05-29T20:34:16Z
dc.date.available 2013-05-29T20:34:16Z
dc.date.issued 2013-05-29
dc.identifier.uri http://hdl.handle.net/2097/15862
dc.description.abstract Toll-like receptors (TLRs) are archetypal pattern recognition receptors of immediate importance for an efficacious innate immune response. TLRs exhibit marked differential tissue activity and their levels within a discrete cell type can be highly dynamic. Of 13 known mammalian paralogues, three TLRs have been identified in the dog. Although cardiac TLR expression has been reported in other species, this study is the first to present evidence that these innate immune receptors are expressed in the canine heart. Heart tissue samples from all four chambers were collected from healthy dogs immediately after euthanasia and stored at -80°C until analysis. Total RNA was extracted with TRI Regent. Specific primers were designed for amplification of canine TLR2 and TLR4 based on previously reported sequences for these genes. Reverse transcription was performed with M-MLV reverse transcriptase. PCR amplification was performed and PCR products analyzed by agarose gel electrophoresis. Bands were excised from the gel and the DNA isolated and cloned using the TA Cloning® Kit. The correct sequence for each product was verified by nucleotide sequencing. TLR4 expression was detected in the left ventricle and right atrium; TLR2 was detectable at low levels in the right atrium only. Identity of the RT-PCR products was confirmed by sequencing. Our findings show that at least two TLR paralogues- namely TLR2 and TLR4 - are expressed in the canine heart. Additional studies are warranted to determine these immune receptors' potential implication in the development of naturally occurring heart disease in the dog. en_US
dc.language.iso en_US en_US
dc.relation.uri http://thescipub.com/abstract/10.3844/ajavsp.2007.6.10 en_US
dc.subject Innate immunity en_US
dc.subject Pattern recognition receptors en_US
dc.subject Cardiac en_US
dc.subject Dog en_US
dc.title Toll-like receptor (TLR) 2 and TLR4 gene expression in canine heart en_US
dc.type Article (publisher version) en_US
dc.date.published 2007 en_US
dc.citation.doi doi:10.3844/ajavsp.2007.6.10 en_US
dc.citation.epage 10 en_US
dc.citation.issue 1 en_US
dc.citation.jtitle American Journal of Animal and Veterinary Sciences en_US
dc.citation.spage 6 en_US
dc.citation.volume 2 en_US
dc.contributor.authoreid alinde en_US
dc.contributor.authoreid blecha en_US
dc.contributor.authoreid tmelgare en_US


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