Characterization of Phlebotomus papatasi peritrophins, and the role of PpPer1 in Leishmania major survival in its natural vector

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Show simple item record Coutinho-Abreu, Iliano V. Sharma, Narinder K. Robles-Murguia, Maricela Ramalho-Ortigão, Marcelo 2013-04-30T22:00:02Z 2013-04-30T22:00:02Z 2013-04-30
dc.description.abstract The peritrophic matrix (PM) plays a key role in compartmentalization of the blood meal and as barrier to pathogens in many disease vectors. To establish an infection in sand flies, Leishmania must escape from the endoperitrophic space to prevent excretion with remnants of the blood meal digestion. In spite of the role played regarding Leishmania survival, little is known about sand fly PM molecular components and structural organization. We characterized three peritrophins (PpPer1, PpPer2, and PpPer3) from Phlebotomus papatasi. PpPer1 and PpPer2 display, respectively, four and one chitin-binding domains (CBDs). PpPer3 on the other hand has two CBDs, one mucin-like domain, and a putative domain with hallmarks of a CBD, but with changes in key amino acids. Temporal and spatial expression analyses show that PpPer1 is expressed specifically in the female midgut after blood feeding. PpPer2 and PpPer3 mRNAs were constitutively expressed in midgut and hindgut, with PpPer3 also being expressed in Malpighian tubules. PpPer2 was the only gene expressed in developmental stages. Interestingly, PpPer1 and PpPer3 expression are regulated by Le. major infection. Recombinant PpPer1, PpPer2 and PpPer3 were obtained and shown to display similar biochemical profiles as the native; we also show that PpPer1 and PpPer2 are able to bind chitin. Knockdown of PpPer1 led to a 44% reduction in protein, which in spite of producing an effect on the percentage of infected sand flies, resulted in a 39% increase of parasite load at 48 h. Our data suggest that PpPer1 is a component for the P. papatasi PM and likely involved in the PM role as barrier against Le. major infection. en_US
dc.language.iso en_US en_US
dc.relation.uri en_US
dc.subject Peritrophic matrix en_US
dc.subject Disease vectors en_US
dc.subject Phlebotomus papatasi en_US
dc.title Characterization of Phlebotomus papatasi peritrophins, and the role of PpPer1 in Leishmania major survival in its natural vector en_US
dc.type Article (publisher version) en_US 2013 en_US
dc.citation.doi doi:10.1371/journal.pntd.0002132 en_US
dc.citation.issue 3 en_US
dc.citation.jtitle PLOS Neglected Tropical Diseases en_US
dc.citation.spage e2132 en_US
dc.citation.volume 7 en_US
dc.contributor.authoreid mortigao en_US

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