Development of a multiplex fluorescent immunoassay for the simultaneous detection of serum antibodies to multiple swine pathogens

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dc.contributor.author Wang, Yu
dc.date.accessioned 2013-04-30T17:24:50Z
dc.date.available 2013-04-30T17:24:50Z
dc.date.issued 2013-04-30
dc.identifier.uri http://hdl.handle.net/2097/15704
dc.description.abstract Three economically important swine diseases: Porcine Reproductive and Respiratory Syndrome (PRRS), Porcine Circovirus Associated Disease (PCVAD) and Swine influenza cost the US swine industry more than a billion dollars each year. This study developed a fluorescent microsphere immunoassay (FMIA) to simultaneously detect antibodies to the causative pathogens: PRRSV, porcine circovirus (PCV2) and swine influenza virus (SIV). The results showed that the multiplex assay possessed the predicted specificities. In the case of PRRSV NA, the assay displayed higher sensitivity when compared to a commercially available ELISA. The assay was employed to measure both IgG and IgM responses. The FMIA was found to possess several advantages over standard ELISA which include reduced sample volume, time and cost and provides a new tool for veterinary diagnostics. The FMIA was applied for swine disease surveillance in Hawaiian and Texan feral swine populations. The antibodies against PCV2 showed the highest prevalence among these three pathogens in both Hawaii and Texas. Hence we consider PCV2 as the most prevalent pathogen in Hawaiian and Texan feral pigs and this pathogen poses the greatest threat to commercial pigs. SIV seroprevelance increased from 2007 to 2010 in Hawaii State, suggesting an increasing risk for commercial pigs. Moreover, yearly surveillance in Texas State shows growth in seropositive response to all pathogens, particularly PCV2. The development of FMIA for detection of antibodies to multiple swine pathogens in serum samples offers an important alternative for swine disease surveillance in commercial and feral herds. en_US
dc.language.iso en_US en_US
dc.publisher Kansas State University en
dc.subject Swine pathogens en_US
dc.subject Immunoassay en_US
dc.subject Luminex en_US
dc.title Development of a multiplex fluorescent immunoassay for the simultaneous detection of serum antibodies to multiple swine pathogens en_US
dc.type Thesis en_US
dc.description.degree Master of Science en_US
dc.description.level Masters en_US
dc.description.department Department of Diagnostic Medicine and Pathobiology en_US
dc.description.advisor Raymond R. R. Rowland en_US
dc.subject.umi Veterinary Medicine (0778) en_US
dc.date.published 2013 en_US
dc.date.graduationmonth May en_US


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