Detoxification and nutritional enhancement of soy meal via microbial bioprocessing

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Show simple item record Chen, Liyan 2013-04-26T13:53:35Z 2013-04-26T13:53:35Z 2013-04-26
dc.description.abstract Soy meal (SM) is the main protein source for monogastric animals. Anti-nutritional factors in SM limited its usage for young monogastric animals. Aspergillus was investigated to degrade these factors and to enhance its nutritional value via solid state fermentation. Galacto-oligosaccharides were totally degraded from the initial 9.48 mmol/100 g, and trypsin inhibitor decreased from 10.7 TIU/mg to a non-detectable level after 36 hr fermentation. Structural polysaccharides decreased by 59% (w/w) and the degree of hydrolysis of SM protein increased from 0.9% to 7% (w/w) through the 7 d fermentation. Fermentation also modified nutritional factors. Protein content increased from 50.47% (w/w) to 58.93% (w/w) after 36 hr fermentation. Amino acid profile was significantly enhanced. Two - stage temperature-induced fermentation protocol was developed to increase the degradation rate of phytic acid by A. oryzae (ATCC 9362) and by A. ficuum (ATCC 66876). The first stage maximized phytase production with fermentation parameters obtained by central composite design. The second stage achieved maximum enzymatic degradation with parameters obtained by studying the phytase temperature characteristics. While using A. oryzae, 57% of phytic acid in SM was degraded by the two stage protocol compared to 39% degradation from single stage fermentation. For A. ficuum, the two-stage temperature fermentation protocol achieved a 98% degradation level of phytic acid degradation compared with the single stage process. Two-stage temperature-induced co-fermentation of A. oryzae and A. ficuum was investigated to simultaneously degrade phytic acid and soy protein with high efficiency. Co-fermentation of A. oryzae and A. ficuum resulted in higher phytic acid degradation than A. oryzae fermentation and superior protein hydrolysis compared to A. ficuum fermentation. Sterilization distorted the results of fermentation effect on soy allergens and soy protein degradation. Virginiamycin is a kind of bacterisin. It was added to A. oryzae solid state fermentation, to exclude the necessity of SM sterilization. Nonsterile, solid state fermentation using A. oryzae and virginiamycin showed the complete degradation of α and α’ subunits of β-conglycinin and decreased immunoreactivity of soy protein. The modified SM after microbial bioprocessing created an innovative product with enhanced characteristics with potential wider applications for feed industry. en_US
dc.language.iso en_US en_US
dc.publisher Kansas State University en
dc.subject Soy meal en_US
dc.subject Fermentation en_US
dc.subject Aspergillus oryzae en_US
dc.subject Aspergillus ficuum en_US
dc.subject Phytic acid en_US
dc.subject Animal feed en_US
dc.title Detoxification and nutritional enhancement of soy meal via microbial bioprocessing en_US
dc.type Dissertation en_US Doctor of Philosophy en_US
dc.description.level Doctoral en_US
dc.description.department Department of Grain Science and Industry en_US
dc.description.advisor Praveen V. Vadlani en_US
dc.subject.umi Agriculture, General (0473) en_US 2013 en_US May en_US

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