Effect of norepinephrine on conjugation of Escherichia coli strains

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dc.contributor.author Alhaadi, Marai
dc.date.accessioned 2013-04-18T18:18:15Z
dc.date.available 2013-04-18T18:18:15Z
dc.date.issued 2013-04-18
dc.identifier.uri http://hdl.handle.net/2097/15527
dc.description.abstract Antibiotics are substances produced by bacteria or fungi that are inhibitory to other bacteria and fungi. Antimicrobial compounds include substances that are naturally produced, chemically modified or completely synthetic (chemically designed or synthesized). The chemical modification of naturally produced antibiotic generally results in increase stability, solubility, increased spectrum of activity, or efficacy. Antimicrobial compounds are used in animals to treat and control infectious diseases, and also for growth promotion. Bacteria may gain resistance to antibacterial agents via a variety of mechanisms. There is growing evidence that antimicrobial resistance has significant public health consequences. Rationale use of antimicrobial drugs using appropriate medication at the proper dosage and for duration is one of the important means to reduce selective pressure that helps reduce life of resistant organism. It is also vital to reduce the spread of multi drug resistant organisms in the environment especially in health care facilities. Bacteria evolve rapidly not only by mutation, but also by horizontal gene transfer through the transformation, transduction, and conjugation. Conjugation involves a close contact between two bacteria and transfer of the plasmid that carry many genetic elements. The pathogenic bacteria have the ability to sense as well as respond to the stress in the recipient. The epinephrine and norepinephrine play a key role in stress situations in animals. A previous study showed that norepinephrine (NE), a catecholamine at physiological concentrations promoted the conjugation efficiencies of a conjugative plasmid from a clinical strain of Salmonella typhimurium to an E. coli recipient in vitro. The objective of this study was to determine the effect of norepinephrine on conjugation of two E. coli strains. Both filter mating and liquid mating assays were used. The results revealed that there was no significance difference between the presence and the absence of norepinephrine on conjugative transfer of RP4 plasmid between E. coli strains (FS1290 and C600N) either in filter mating or liquid mating. Further studies are needed to determine whether higher concentration of (more than 20 mM) has any effects on conjugation in E. coli. en_US
dc.description.sponsorship Kansas State University en_US
dc.language.iso en_US en_US
dc.publisher Kansas State University en
dc.subject Antimicrobial compounds en_US
dc.subject Conjugation en_US
dc.subject E. coli en_US
dc.subject Norepinephrine en_US
dc.title Effect of norepinephrine on conjugation of Escherichia coli strains en_US
dc.type Report en_US
dc.description.degree Master of Science en_US
dc.description.level Masters en_US
dc.description.department Department of Diagnostic Medicine/Pathobiology en_US
dc.description.advisor Sanjeev K. Narayanan en_US
dc.subject.umi Veterinary Medicine (0778) en_US
dc.date.published 2013 en_US
dc.date.graduationmonth May en_US

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