Characterization and functional analysis of four glutathione S-transferases from the migratory locust, Locusta migratoria

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dc.contributor.author Qin, Guohua
dc.contributor.author Jia, Miao
dc.contributor.author Liu, Ting
dc.contributor.author Zhang, Xueyao
dc.contributor.author Guo, Yaping
dc.contributor.author Zhu, Kun Yan
dc.contributor.author Ma, Enbo
dc.contributor.author Zhang, Jianzhen
dc.date.accessioned 2013-04-11T13:47:55Z
dc.date.available 2013-04-11T13:47:55Z
dc.date.issued 2013-04-11
dc.identifier.uri http://hdl.handle.net/2097/15491
dc.description.abstract Glutathione S-transferases (GSTs) play an important role in detoxification of xenobiotics in both prokaryotic and eukaryotic cells. In this study, four GSTs (LmGSTd1, LmGSTs5, LmGSTt1, and LmGSTu1) representing different classes were identified from the migratory locust, Locusta migratoria. These four proteins were heterologously expressed in Escherichia coli as soluble fusion proteins, purified by Ni[superscript 2+]-nitrilotriacetic acid agarose column and biochemically characterized. LmGSTd1, LmGSTs5, and LmGSTu1 showed high activities with 1-chloro-2, 4-dinitrobenzene (CDNB), detectable activity with p-nitro-benzyl chloride (p-NBC) and 1, 2-dichloro-4-nitrobenzene (DCNB), whereas LmGSTt1 showed high activity with p-NBC and detectable activity with CDNB. The optimal pH of the locust GSTs ranged between 7.0 to 9.0. Ethacrynic acid and reactive blue effectively inhibited all four GSTs. LmGSTs5 was most sensitive to heavy metals (Cu[superscript 2+] and Cd[superscript 2+]). The maximum expression of the four GSTs was observed in Malpighian tubules and fat bodies as evaluated by western blot. The nymph mortalities after carbaryl treatment increased by 28 and 12% after LmGSTs5 and LmGSTu1 were silenced, respectively. The nymph mortalities after malathion and chlorpyrifos treatments increased by 26 and 18% after LmGSTs5 and LmGSTu1 were silenced, respectively. These results suggest that sigma GSTs in L. migratoria play a significant role in carbaryl detoxification, whereas some of other GSTs may also involve in the detoxification of carbaryl and chlorpyrifos. en_US
dc.language.iso en_US en_US
dc.relation.uri http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0058410 en_US
dc.subject Glutathione S-transferases en_US
dc.subject Migratory Locust en_US
dc.subject Locusta migratoria en_US
dc.title Characterization and functional analysis of four glutathione S-transferases from the migratory locust, Locusta migratoria en_US
dc.type Article (publisher version) en_US
dc.date.published 2013 en_US
dc.citation.doi doi:10.1371/journal.pone.0058410 en_US
dc.citation.issue 3 en_US
dc.citation.jtitle PLoS ONE en_US
dc.citation.spage e58410 en_US
dc.citation.volume 8 en_US
dc.contributor.authoreid kzhu en_US


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