Infection of Aedes albopictus with chikungunya virus rectally administered by enema

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dc.contributor.author Nuckols, John T.
dc.contributor.author Ziegler, Sarah A.
dc.contributor.author Huang, Yan-Jang Scott
dc.contributor.author McAuley, Alex J.
dc.contributor.author Vanlandingham, Dana L.
dc.contributor.author Klowden, Marc J.
dc.contributor.author Spratt, Heidi
dc.contributor.author Davey, Robert A.
dc.contributor.author Higgs, Stephen
dc.date.accessioned 2013-03-28T18:43:16Z
dc.date.available 2013-03-28T18:43:16Z
dc.date.issued 2013-03-28
dc.identifier.uri http://hdl.handle.net/2097/15427
dc.description.abstract Chikungunya virus (CHIKV) is an alphavirus transmitted by Aedes albopictus and Aedes aegypti mosquitoes in tropical areas of Africa, Asia, and the islands of the Indian Ocean. In 2007 and 2009, CHIKV was transmitted outside these tropical areas and caused geographically localized infections in people in Italy and France. To temporally and spatially characterize CHIKV infection of Ae. albopictus midguts, a comparison of viral distribution in mosquitoes infected per os or by enema was conducted. Ae. albopictus infected with CHIKV LR 5′ green fluorescent protein (GFP) at a titer 10[superscript 6.95] tissue culture infective dose[subscript 50] (TCID[subscript 50])/mL, were collected and analyzed for virus dissemination by visualizing GFP expression and titration up to 14 days post inoculation (dpi). Additionally, midguts were dissected from the mosquitoes and imaged by fluorescence microscopy for comparison of midgut infection patterns between orally- and enema-infected mosquitoes. When virus was delivered via enema, the anterior midgut appeared more readily infected by 3 dpi, with increased GFP presentation observed in this same location of the midgut at 7 and 14 dpi when compared to orally-infected mosquitoes. This work demonstrates that enema delivery of virus is a viable technique for use of mosquito infection. Enema injection of mosquitoes may be an alternative to intrathoracic inoculation because the enema delivery more closely models natural infection and neither compromises midgut integrity nor involves a wound that can induce immune responses. Furthermore, unlike intrathoracic delivery, the enema does not bypass midgut barriers to infect tissues artificially in the hemocoel of the mosquito. en_US
dc.language.iso en_US en_US
dc.relation.uri http://online.liebertpub.com/doi/full/10.1089/vbz.2012.1013 en_US
dc.rights This is a copy of an article published in the Vector-Borne and Zoonotic Diseases © 2013 copyright Mary Ann Liebert, Inc.; Vector-Borne and Zoonotic Diseases is available online at: http://online.liebertpub.com. en_US
dc.subject Chikungunya virus en_US
dc.subject Aedes albopictus en_US
dc.subject Aedes aegypti en_US
dc.subject Enema injection en_US
dc.title Infection of Aedes albopictus with chikungunya virus rectally administered by enema en_US
dc.type Article (publisher version) en_US
dc.date.published 2013 en_US
dc.citation.doi doi:10.1089/vbz.2012.1013 en_US
dc.citation.epage 110 en_US
dc.citation.issue 2 en_US
dc.citation.jtitle Vector-Borne and Zoonotic Diseases en_US
dc.citation.spage 103 en_US
dc.citation.volume 13 en_US
dc.contributor.authoreid shiggs en_US


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