Impact of Wolbachia on infection with chikungunya and yellow fever viruses in the mosquito vector Aedes aegypti

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dc.contributor.author Van den Hurk, Andrew F.
dc.contributor.author Hall-Mendelin, Sonja
dc.contributor.author Pyke, Alyssa T.
dc.contributor.author Frentiu, Francesca D.
dc.contributor.author McElroy, Kate
dc.contributor.author Day, Andrew
dc.contributor.author Higgs, Stephen
dc.contributor.author O’Neill, Scott L.
dc.date.accessioned 2013-03-22T14:59:24Z
dc.date.available 2013-03-22T14:59:24Z
dc.date.issued 2013-03-22
dc.identifier.uri http://hdl.handle.net/2097/15399
dc.description.abstract Incidence of disease due to dengue (DENV), chikungunya (CHIKV) and yellow fever (YFV) viruses is increasing in many parts of the world. The viruses are primarily transmitted by Aedes aegypti, a highly domesticated mosquito species that is notoriously difficult to control. When transinfected into Ae. aegypti, the intracellular bacterium Wolbachia has recently been shown to inhibit replication of DENVs, CHIKV, malaria parasites and filarial nematodes, providing a potentially powerful biocontrol strategy for human pathogens. Because the extent of pathogen reduction can be influenced by the strain of bacterium, we examined whether the wMel strain of Wolbachia influenced CHIKV and YFV infection in Ae. aegypti. Following exposure to viremic blood meals, CHIKV infection and dissemination rates were significantly reduced in mosquitoes with the wMel strain of Wolbachia compared to Wolbachia-uninfected controls. However, similar rates of infection and dissemination were observed in wMel infected and non-infected Ae. aegypti when intrathoracic inoculation was used to deliver virus. YFV infection, dissemination and replication were similar in wMel-infected and control mosquitoes following intrathoracic inoculations. In contrast, mosquitoes with the wMelPop strain of Wolbachia showed at least a 104 times reduction in YFV RNA copies compared to controls. The extent of reduction in virus infection depended on Wolbachia strain, titer and strain of the virus, and mode of exposure. Although originally proposed for dengue biocontrol, our results indicate a Wolbachia-based strategy also holds considerable promise for YFV and CHIKV suppression. en_US
dc.language.iso en_US en_US
dc.relation.uri http://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0001892 en_US
dc.subject Chikungunya virus en_US
dc.subject Yellow fever virus en_US
dc.subject Aedes aegypti en_US
dc.subject Wolbachia en_US
dc.subject Mosquitoes en_US
dc.title Impact of Wolbachia on infection with chikungunya and yellow fever viruses in the mosquito vector Aedes aegypti en_US
dc.type Article (publisher version) en_US
dc.date.published 2012 en_US
dc.citation.doi doi:10.1371/journal.pntd.0001892 en_US
dc.citation.issue 11 en_US
dc.citation.jtitle PLoS Neglected Tropical Diseases en_US
dc.citation.spage e1892 en_US
dc.citation.volume 6 en_US
dc.contributor.authoreid shiggs en_US


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