Factors affecting sperm recovery rates and survival after centrifugation of equine semen

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dc.contributor.author Ferrer, Maria S.
dc.contributor.author Lyle, S. K.
dc.contributor.author Eilts, B. E.
dc.contributor.author Eljarrah, A. H.
dc.contributor.author Paccamonti, D. L.
dc.date.accessioned 2013-01-30T14:48:07Z
dc.date.available 2013-01-30T14:48:07Z
dc.date.issued 2012-11-01
dc.identifier.uri http://hdl.handle.net/2097/15278
dc.description.abstract Conventional centrifugation protocols result in important sperm losses during removal of the supernatant. In this study, the effect of centrifugation force (400 or 900 × g), duration (5 or 10 min), and column height (20 or 40 mL; Experiment 1); sperm concentration (25, 50, and 100 × 10[superscript 6]/mL; Experiment 2), and centrifugation medium (EZ-Mixin CST [Animal Reproduction Systems, Chino, CA, USA], INRA96 [IMV Technologies, Maple Grove, MN, USA], or VMDZ [Partnar Animal Health, Port Huron, MI, USA]; Experiment 3) on sperm recovery and survival after centrifugation and cooling and storage were evaluated. Overall, sperm survival was not affected by the combination of centrifugation protocol and cooling. Total sperm yield was highest after centrifugation for 10 min at 400 × g in 20-mL columns (95.6 ± 5%, mean ± SD) or 900 × g in 20-mL (99.2 ± 0.8%) or 40-mL (91.4 ± 4.5%) columns, and at 900 × g for 5 min in 20-mL columns (93.8 ± 8.9%; P < 0.0001). Total (TMY) and progressively motile sperm yield followed a similar pattern (P < 0.0001). Sperm yields were not significantly different among samples centrifuged at various sperm concentrations. However, centrifugation at 100 × 10[superscript 6]/mL resulted in significantly lower total sperm yield (83.8 ± 10.7%) and TMY (81.7 ± 6.8%) compared with noncentrifuged semen. Centrifugation in VMDZ resulted in significantly lower TMY (69.3 ± 22.6%), progressively motile sperm yield (63.5 ± 18.2%), viable yield (60.9 ± 36.5%), and survival of progressively motile sperm after cooling (21 ± 10.8%) compared with noncentrifuged semen. In conclusion, centrifuging volumes of ≤ 20 mL minimized sperm losses with conventional protocols. With 40-mL columns, it may be recommended to increase the centrifugal force to 900 × g for 10 min and dilute the semen to a sperm concentration of 25 to 50 × 10[superscript 6]/mL in a milk- or fractionated milk-based medium. The semen extender VMDZ did not seem well suited for centrifugation of equine semen. en_US
dc.language.iso en_US en_US
dc.relation.uri http://www.doi.org/10.1016/j.theriogenology.2012.07.011 en_US
dc.subject Centrifugation en_US
dc.subject Stallion semen en_US
dc.subject Semen en_US
dc.subject Semen viability en_US
dc.subject Recovery en_US
dc.subject Horse en_US
dc.title Factors affecting sperm recovery rates and survival after centrifugation of equine semen en_US
dc.type Article (author version) en_US
dc.date.published 2012 en_US
dc.citation.doi 10.1016/j.theriogenology.2012.07.011 en_US
dc.citation.epage 1823 en_US
dc.citation.issue 8 en_US
dc.citation.jtitle Theriogenology en_US
dc.citation.spage 1814 en_US
dc.citation.volume 78 en_US
dc.contributor.authoreid mferrer en_US

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