Gene families of cuticular proteins analogous to peritrophins (CPAPs) in Tribolium castaneum have diverse functions

K-REx Repository

Show simple item record

dc.contributor.author Jasrapuria, Sinu
dc.contributor.author Specht, Charles A.
dc.contributor.author Kramer, Karl J.
dc.contributor.author Beeman, Richard W.
dc.contributor.author Muthukrishnan, Subbaratnam
dc.date.accessioned 2013-01-22T17:36:28Z
dc.date.available 2013-01-22T17:36:28Z
dc.date.issued 2013-01-22
dc.identifier.uri http://hdl.handle.net/2097/15225
dc.description Citation: Jasrapuria S, Specht CA, Kramer KJ, Beeman RW, Muthukrishnan S (2012) Gene Families of Cuticular Proteins Analogous to Peritrophins (CPAPs) in Tribolium castaneum Have Diverse Functions. PLoS ONE 7(11): e49844. doi:10.1371/journal.pone.0049844
dc.description.abstract The functional characterization of an entire class of 17 genes from the red flour beetle, Tribolium castaneum, which encode two families of Cuticular Proteins Analogous to Peritrophins (CPAPs) has been carried out. CPAP genes in T. castaneum are expressed exclusively in cuticle-forming tissues and have been classified into two families, CPAP1 and CPAP3, based on whether the proteins contain either one (CPAP1), or three copies (CPAP3) of the chitin-binding domain, ChtBD2, with its six characteristically spaced cysteine residues. Individual members of the TcCPAP1 and TcCPAP3 gene families have distinct developmental patterns of expression. Many of these proteins serve essential and non-redundant functions in maintaining the structural integrity of the cuticle in different parts of the insect anatomy. Three genes of the TcCPAP1 family and five genes of the TcCPAP3 family are essential for insect development, molting, cuticle integrity, proper locomotion or fecundity. RNA interference (RNAi) targeting TcCPAP1-C, TcCPAP1-H, TcCPAP1-J or TcCPAP3-C transcripts resulted in death at the pharate adult stage of development. RNAi for TcCPAP3-A1, TcCPAP3-B, TcCPAP3-D1 or TcCPAP3-D2 genes resulted in different developmental defects, including adult/embryonic mortality, abnormal elytra or hindwings, or an abnormal ‘stiff-jointed’ gait. These results provide experimental support for specialization in the functions of CPAP proteins in T. castaneum and a biological rationale for the conservation of CPAP orthologs in other orders of insects. This is the first comprehensive functional analysis of an entire class of cuticular proteins with one or more ChtBD2 domains in any insect species. en_US
dc.language.iso en_US en_US
dc.relation.uri https://doi.org/10.1371/journal.pone.0049844 en_US
dc.rights Attribution 4.0 International (CC BY 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Tribolium castaneum en_US
dc.subject Red flour beetle en_US
dc.subject Cuticular Proteins Analogous to Peritrophins en_US
dc.subject CPAPs en_US
dc.subject Chitin en_US
dc.subject Cuticular proteins en_US
dc.title Gene families of cuticular proteins analogous to peritrophins (CPAPs) in Tribolium castaneum have diverse functions en_US
dc.type Article (publisher version) en_US
dc.date.published 2012 en_US
dc.citation.doi 10.1371/journal.pone.0049844 en_US
dc.citation.issue 11 en_US
dc.citation.jtitle PLoS ONE en_US
dc.citation.volume 7 en_US
dc.citation Jasrapuria S, Specht CA, Kramer KJ, Beeman RW, Muthukrishnan S (2012) Gene Families of Cuticular Proteins Analogous to Peritrophins (CPAPs) in Tribolium castaneum Have Diverse Functions. PLoS ONE 7(11): e49844. doi:10.1371/journal.pone.0049844
dc.contributor.authoreid kjkramer en_US
dc.contributor.authoreid smk en_US
dc.contributor.authoreid rwbeeman


Files in this item

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International (CC BY 4.0) Except where otherwise noted, the use of this item is bound by the following: Attribution 4.0 International (CC BY 4.0)

Search K-REx


Advanced Search

Browse

My Account

Statistics








Center for the

Advancement of Digital

Scholarship

cads@k-state.edu