Transcriptome profiling of the intoxication response of Tenebrio molitor larvae to Bacillus thuringiensis Cry3Aa protoxin

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dc.contributor.author Oppert, Brenda
dc.contributor.author Dowd, Scot E.
dc.contributor.author Bouffard, Pascal
dc.contributor.author Li, Lewyn
dc.contributor.author Conesa, Ana
dc.contributor.author Lorenzen, Marce´ D.
dc.contributor.author Toutges, Michelle
dc.contributor.author Marshall, Jeremy
dc.contributor.author Huestis, Diana L.
dc.contributor.author Fabrick, Jeff
dc.contributor.author Oppert, Cris
dc.contributor.author Jurat- Fuentes, Juan Luis
dc.date.accessioned 2012-07-24T19:27:12Z
dc.date.available 2012-07-24T19:27:12Z
dc.date.issued 2012-07-24
dc.identifier.uri http://hdl.handle.net/2097/14078
dc.description Citation: Oppert B, Dowd SE, Bouffard P, Li L, Conesa A, et al. (2012) Transcriptome Profiling of the Intoxication Response of Tenebrio molitor Larvae to Bacillus thuringiensis Cry3Aa Protoxin. PLoS ONE 7(4): e34624. doi:10.1371/journal.pone.0034624
dc.description.abstract Bacillus thuringiensis (Bt) crystal (Cry) proteins are effective against a select number of insect pests, but improvements are needed to increase efficacy and decrease time to mortality for coleopteran pests. To gain insight into the Bt intoxication process in Coleoptera, we performed RNA-Seq on cDNA generated from the guts of Tenebrio molitor larvae that consumed either a control diet or a diet containing Cry3Aa protoxin. Approximately 134,090 and 124,287 sequence reads from the control and Cry3Aa-treated groups were assembled into 1,318 and 1,140 contigs, respectively. Enrichment analyses indicated that functions associated with mitochondrial respiration, signalling, maintenance of cell structure, membrane integrity, protein recycling/synthesis, and glycosyl hydrolases were significantly increased in Cry3Aa-treated larvae, whereas functions associated with many metabolic processes were reduced, especially glycolysis, tricarboxylic acid cycle, and fatty acid synthesis. Microarray analysis was used to evaluate temporal changes in gene expression after 6, 12 or 24 h of Cry3Aa exposure. Overall, microarray analysis indicated that transcripts related to allergens, chitin-binding proteins, glycosyl hydrolases, and tubulins were induced, and those related to immunity and metabolism were repressed in Cry3Aaintoxicated larvae. The 24 h microarray data validated most of the RNA-Seq data. Of the three intoxication intervals, larvae demonstrated more differential expression of transcripts after 12 h exposure to Cry3Aa. Gene expression examined by three different methods in control vs. Cry3Aa-treated larvae at the 24 h time point indicated that transcripts encoding proteins with chitin-binding domain 3 were the most differentially expressed in Cry3Aa-intoxicated larvae. Overall, the data suggest that T. molitor larvae mount a complex response to Cry3Aa during the initial 24 h of intoxication. Data from this study represent the largest genetic sequence dataset for T. molitor to date. Furthermore, the methods in this study are useful for comparative analyses in organisms lacking a sequenced genome. en_US
dc.relation.uri https://doi.org/10.1371/journal.pone.0034624 en_US
dc.rights This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
dc.rights.uri https://creativecommons.org/publicdomain/zero/1.0/
dc.subject Bacillus thuringiensis en_US
dc.subject Tenebrio molitor en_US
dc.subject Cry3Aa en_US
dc.subject Transcriptome profiling en_US
dc.title Transcriptome profiling of the intoxication response of Tenebrio molitor larvae to Bacillus thuringiensis Cry3Aa protoxin en_US
dc.type Text en_US
dc.date.published 2012 en_US
dc.citation.doi 10.1371/journal.pone.0034624 en_US
dc.citation.issn 1932-6203
dc.citation.issue 4 en_US
dc.citation.jtitle Plos One en_US
dc.citation.spage e34624 en_US
dc.citation.volume 7 en_US
dc.citation Oppert B, Dowd SE, Bouffard P, Li L, Conesa A, et al. (2012) Transcriptome Profiling of the Intoxication Response of Tenebrio molitor Larvae to Bacillus thuringiensis Cry3Aa Protoxin. PLoS ONE 7(4): e34624. doi:10.1371/journal.pone.0034624
dc.contributor.authoreid bso en_US
dc.contributor.authoreid cricket en_US
dc.description.version Article: Version of Record


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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. Except where otherwise noted, the use of this item is bound by the following: This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

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