Evaluation of macrophage plasticity in brown and white adipose tissue

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dc.contributor.author Ortega, M. Teresa
dc.contributor.author Xie, Linglin
dc.contributor.author Mora, Silvia
dc.contributor.author Chapes, Stephen K.
dc.date.accessioned 2012-07-09T19:50:07Z
dc.date.available 2012-07-09T19:50:07Z
dc.date.issued 2012-07-09
dc.identifier.uri http://hdl.handle.net/2097/14016
dc.description.abstract There are still questions about whether macrophage differentiation is predetermined or is induced in response to tissue microenvironments. C2D macrophage cells reside early in the macrophage lineage in vitro, but differentiate to a more mature phenotype after adoptive transfer to the peritoneal cavity (PEC-C2D). Since C2D macrophage cells also traffic to adipose tissue after adoptive transfer, we explored the impact of white adipose tissue (WAT), brown adipose tissue (BAT) and in vitro cultured adipocytes on C2D macrophage cells. When PEC-C2D macrophage cells were cultured with preadipocytes the cells stretched out and CD11b and Mac-2 expression was lower compared to PEC-C2D macrophage cells placed in vitro alone. In contrast, PEC-C2D cells co-cultured with adipocytes maintained smaller, round morphology and more cells expressed Mac-2 compared to PEC-C2D co-cultured with preadipocytes. After intraperitoneal injection, C2D macrophage cells migrated into both WAT and BAT. A higher percentage of C2D macrophage cells isolated from WAT (WAT-C2D) expressed Ly-6C (33%), CD11b (11%), Mac-2 (11%) and F4/80 (29%) compared to C2D macrophage cells isolated from BAT (BAT-C2D). Overall, BAT-C2D macrophage cells had reduced expression of many cytokine, chemokine and receptor gene transcripts when compared to in vitro grown C2D macrophages, while WAT-C2D macrophage cells and PEC-C2D up-regulated many of these gene transcripts. These data suggest that the C2D macrophage phenotype can change rapidly and distinct phenotypes are induced by different microenvironments. en_US
dc.relation.uri http://www.sciencedirect.com/science/article/pii/S000887491100150X en_US
dc.subject Macrophage en_US
dc.subject Plasticity en_US
dc.subject Adipocyte en_US
dc.subject Adipose tissue en_US
dc.subject Trafficking en_US
dc.title Evaluation of macrophage plasticity in brown and white adipose tissue en_US
dc.type Article (author version) en_US
dc.date.published 2011 en_US
dc.citation.doi doi:10.1016/j.cellimm.2011.06.012 en_US
dc.citation.epage 133 en_US
dc.citation.issue 1 en_US
dc.citation.jtitle Cellular Immunology en_US
dc.citation.spage 124 en_US
dc.citation.volume 271 en_US
dc.contributor.authoreid mto5535 en_US
dc.contributor.authoreid skcbiol en_US

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