Direct infusion mass spectrometry of oxylipin-containing Arabidopsis membrane lipids reveals varied patterns in different stress responses

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dc.contributor.author Vu, Hieu Sy
dc.contributor.author Tamura, Pamela
dc.contributor.author Galeva, Nadezhda A.
dc.contributor.author Chaturvedi, Ratnesh
dc.contributor.author Roth, Mary R.
dc.contributor.author Williams, Todd D.
dc.contributor.author Wang, Xuemin
dc.contributor.author Shah, Jyoti
dc.contributor.author Welti, Ruth
dc.date.accessioned 2012-06-11T20:21:12Z
dc.date.available 2012-06-11T20:21:12Z
dc.date.issued 2012-06-11
dc.identifier.uri http://hdl.handle.net/2097/13926
dc.description.abstract Direct infusion electrospray ionization triple quadrupole precursor scanning for three oxidized fatty acyl anions revealed 86 mass spectral peaks representing polar membrane lipids in extracts from Arabidopsis (Arabidopsis thaliana) infected with Pseudomonas syringae pv tomato DC3000 expressing AvrRpt2 (PstAvr). Quadrupole time-of-flight and Fourier transform ion cyclotron resonance mass spectrometry provided evidence for the presence of membrane lipids containing one or more oxidized acyl chains. The membrane lipids included molecular species of phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, digalactosyldiacylglycerol, monogalactosyldiacylglycerol, and acylated monogalactosyldiacylglycerol. The oxidized chains were identified at the level of chemical formula and included C18H27O3 (abbreviated 18:4-O, to indicate four double bond equivalents and one oxygen beyond the carbonyl group), C18H29O3 (18:3-O), C18H31O3 (18:2-O), C18H29O4 (18:3-2O), C18H31O4 (18:2-2O), and C16H23O3 (16:4-O). Mass spectral signals from the polar oxidized lipid (ox-lipid) species were quantified in extracts of Arabidopsis leaves subjected to wounding, infection by PstAvr, infection by a virulent strain of P. syringae, and low temperature. Ox-lipids produced low amounts of mass spectral signal, 0.1% to 3.2% as much as obtained in typical direct infusion profiling of normal-chain membrane lipids of the same classes. Analysis of the oxidized membrane lipid species and normal-chain phosphatidic acids indicated that stress-induced ox-lipid composition differs from the basal ox-lipid composition. Additionally, different stresses result in the production of varied amounts, different timing, and different compositional patterns of stress-induced membrane lipids. These data form the basis for a working hypothesis that the stress-specific signatures of ox-lipids, like those of oxylipins, are indicative of their functions. en_US
dc.relation.uri http://www.plantphysiol.org/content/158/1/324.full?sid=d8f3d99f-c866-4cb7-a663-b64fcf329cf1 en_US
dc.rights Permission to archive granted by the American Society of Plant Biologists, April 23, 2012. en_US
dc.subject Direct infusion mass spectrometry en_US
dc.subject Arabidopsis thaliana en_US
dc.subject Stress responses en_US
dc.subject Membrane lipids en_US
dc.title Direct infusion mass spectrometry of oxylipin-containing Arabidopsis membrane lipids reveals varied patterns in different stress responses en_US
dc.type Article (publisher version) en_US
dc.date.published 2012 en_US
dc.citation.doi doi:http:/​/​dx.​doi.​org/​10.​1104/​pp.​111.​190280 en_US
dc.citation.epage 339 en_US
dc.citation.issue 1 en_US
dc.citation.jtitle Plant Physiology en_US
dc.citation.spage 324 en_US
dc.citation.volume 158 en_US
dc.contributor.authoreid vusyhieu en_US
dc.contributor.authoreid ptamura en_US
dc.contributor.authoreid mrroth en_US
dc.contributor.authoreid shah en_US
dc.contributor.authoreid welti en_US


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